Immunoprecipitation (IP)
(KYNU was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-KYNU rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KYNU rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 52 kDa)
Rabbit anti-Human kynureninase/KYNU Polyclonal Antibody | anti-KYNU antibody
Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal
Gene Names
KYNU; KYNUU
Reactivity
Human
Applications
Western Blot, ELISA, Immunoprecipitation
Purity
Protein A & Antigen Affinity
Synonyms
kynureninase/KYNU; Polyclonal Antibody; Anti-kynureninase/KYNU Antibody; Rabbit Polyclonal; KYNU Antibody; Rabbit PAb; Antigen Affinity Purified; kynureninase; Anti-KYNUU Antibody; anti-KYNU antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human KYNU
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-KYNU antibody
Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
Application Notes
WB: 1:500-1:2000
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
ELISA: 1:5000-1:10,000
IP: 1-4uL/mg of lysate
Immunogen
Recombinant Human KYNU protein
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with purified, recombinant Human KYNU (rh KYNU). KYNU specific IgG was purified by Human KYNU affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.
Immunoprecipitation (IP)
(KYNU was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-KYNU rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KYNU rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 52 kDa)
Immunoprecipitation (IP)
(KYNU was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell Lysate4 uL anti-KYNU rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-KYNU rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Clean-Blot IP Detection Reagent (HRP) at 1:1000 dilution Developed using the DAB staining technique.Performed under reducing conditions.Predicted band size: 52 kDaObserved band size: 52 kDa)
Western Blot (WB)
(Anti-KYNU rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDaObserved band size:52 kDa(We are unsure as to the identity of these extra bands.))
Western Blot (WB)
(Anti-KYNU rabbit polyclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:52 kDaObserved band size:52 kDa(We are unsure as to the identity of these extra bands.))
Related Product Information for anti-KYNU antibody
KYNU (Kynureninase) is a Protein Coding gene. KYNU is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Genetic studies in the mouse and the human suggest that kynureninase activity may influence blood pressure and renal function. The gene coding kynureninase (KYNU) is also located on chromosome band 2q14-q23, where a linkage peak for essential hypertension was previously detected in the Chinese Han population. The results show that the rare KYNU variant Arg188Gln affects kynureninase activity and are consistent with the hypothesis that this mutation can predispose to essential hypertension. Diseases associated with KYNU include Hydroxykynureninuria and Vertebral, Cardiac, Renal, And Limb Defects Syndrome 2.
References
Lima S, et al. (2007) Crystal structure of Homo sapiens kynureninase. Biochemistry. 46(10): 2735-44.Momany C, et al. (2004) Three-dimensional structure of kynureninase from Pseudomonas fluorescens. Biochemistry. 43(5): 1193-203.Toma S, et al. (1997) Cloning and recombinant expression of rat and human kynureninase. FEBS Lett. 408(1): 5-10.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
34,635 Da
NCBI Official Full Name
kynureninase isoform a
NCBI Official Synonym Full Names
kynureninase
NCBI Official Symbol
KYNU
NCBI Official Synonym Symbols
KYNUU
NCBI Protein Information
kynureninase; KYNU; L-kynurenine hydrolase
UniProt Protein Name
Kynureninase
UniProt Gene Name
KYNU
UniProt Entry Name
KYNU_HUMAN
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Product Notes
The KYNU kynu (Catalog #AAA8104147) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-kynureninase/KYNU Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's kynureninase/KYNU can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP). WB: 1:500-1:2000 ELISA: 1:5000-1:10,000 IP: 1-4uL/mg of lysate. Researchers should empirically determine the suitability of the KYNU kynu for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "kynureninase/KYNU, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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