Rabbit anti-Human KYNU Polyclonal Antibody | anti-KYNU antibody

KYNU, CT (Kynureninase, L-kynurenine Hydrolase) (MaxLight 650)

Cat. Num. AAA6484599

• Gene Names: KYNU; KYNUU; VCRL2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: KYNU; Polyclonal Antibody; CT (Kynureninase; L-kynurenine Hydrolase) (MaxLight 650); EC=3.7.1.3; anti-KYNU antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KYNU.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-KYNU antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:50-1:100; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human KYNU (KLH).
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KYNU Antibody (C-term) in NCI-H460 cell line lysates (35ug/lane). KYNU (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with KYNU Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KYNU Antibody (C-term) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KYNU antibody

Antibodies; Enzymes

References

1. Christensen, M., et.al., J. Inherit. Metab. Dis. 30(2), 248-255 (2007).

NCBI and Uniprot Product Information

• NCBI GI #: 4504937
• NCBI GeneID: 8942
• NCBI Accession #: NP_003928
• NCBI GenBank Nucleotide #: NM_003937
• UniProt Accession #: Q16719
• Molecular Weight: 52kDa
• NCBI Official Full Name: kynureninase isoform a
• NCBI Official Synonym Full Names: kynureninase
• NCBI Official Symbol: KYNU
• NCBI Official Synonym Symbols: KYNUU; VCRL2
• NCBI Protein Information: kynureninase
• UniProt Protein Name: Kynureninase
• Protein Family: Kynureninase
• UniProt Gene Name: KYNU
• UniProt Entry Name: KYNU_HUMAN

NCBI Description

Kynureninase is a pyridoxal-5'-phosphate (pyridoxal-P) dependent enzyme that catalyzes the cleavage of L-kynurenine and L-3-hydroxykynurenine into anthranilic and 3-hydroxyanthranilic acids, respectively. Kynureninase is involved in the biosynthesis of NAD cofactors from tryptophan through the kynurenine pathway. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2010]