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Western Blot (WB) (Western blot analysis of Kv3.4/KCNC4 (Phospho-Ser15) using TNF-α treated HeLa whole cell lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)

Rabbit Kv3.4/KCNC4 Polyclonal Antibody | anti-Kv3.4/KCNC4 antibody

Phospho-Kv3.4/KCNC4 (Ser15) Antibody

Gene Names
KCNC4; KV3.4; C1orf30; KSHIIIC; HKSHIIIC
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%)
Applications
Western Blot, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
Kv3.4/KCNC4; Polyclonal Antibody; Phospho-Kv3.4/KCNC4 (Ser15) Antibody; anti-Kv3.4/KCNC4 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-Kv3.4/KCNC4 (Ser15) Antibody detects endogenous levels of Kv3.4/KCNC4 only when phosphorylated at Ser15.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Kv3.4/KCNC4 antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Kv3.4/KCNC4 around the phosphorylation site of Ser15.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylation of serine residues in the inactivation gate inhibits rapid channel closure.
Subunit Structure
Homotetramer (Probable). Heterotetramer of potassium channel proteins (By similarity).
Similarity
The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.The tail may be important in modulation of channel activity and/or targeting of the channel to specific subcellular compartments.Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.4/KCNC4 sub-subfamily.
Subcellular Location
Membrane>Multi-pass membrane protein.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of Kv3.4/KCNC4 (Phospho-Ser15) using TNF-α treated HeLa whole cell lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)

Western Blot (WB) (Western blot analysis of Kv3.4/KCNC4 (Phospho-Ser15) using TNF-α treated HeLa whole cell lysates.-/+ means absence or presence of N peptide(non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-Kv3.4/KCNC4 antibody
This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
69,767 Da
NCBI Official Full Name
potassium voltage-gated channel subfamily C member 4 isoform c
NCBI Official Synonym Full Names
potassium voltage-gated channel, Shaw-related subfamily, member 4
NCBI Official Symbol
KCNC4
NCBI Official Synonym Symbols
KV3.4; C1orf30; KSHIIIC; HKSHIIIC
NCBI Protein Information
potassium voltage-gated channel subfamily C member 4; K+ channel subunit; voltage-gated potassium channel subunit KV3.4
UniProt Protein Name
Potassium voltage-gated channel subfamily C member 4
UniProt Gene Name
KCNC4
UniProt Entry Name
KCNC4_HUMAN

NCBI Description

The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. It generates atypical voltage-dependent transient current that may be important for neuronal excitability. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]

Uniprot Description

Kv3.4: This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.4/KCNC4 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Channel, potassium; Membrane protein, integral

Chromosomal Location of Human Ortholog: 1p21

Cellular Component: voltage-gated potassium channel complex; plasma membrane; integral to membrane; nerve terminal; neuromuscular junction

Molecular Function: voltage-gated potassium channel activity; potassium channel activity; delayed rectifier potassium channel activity

Biological Process: synaptic transmission; regulation of neurotransmitter secretion; protein homooligomerization; potassium ion transport

Research Articles on Kv3.4/KCNC4

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Product Notes

The Kv3.4/KCNC4 kcnc4 (Catalog #AAA9614074) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Kv3.4/KCNC4 (Ser15) Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Dog (100%), Chicken (100%) and may cross-react with other species as described in the data sheet. AAA Biotech's Kv3.4/KCNC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:1000-3000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the Kv3.4/KCNC4 kcnc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kv3.4/KCNC4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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