Western Blot (WB)
(Western Blot (WB) analysis of KV3. 4 (R11) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Rabbit Kv3.4 Polyclonal Antibody | anti-Kv3.4 antibody
Kv3.4 (R11) Polyclonal Antibody
IHC: 1:50-1:200
IF: 1:50-1:200
IP: 1:10-1:100
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot (WB) analysis of KV3. 4 (R11) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Western Blot (WB)
(Western Blot (WB) analysis of KV3. 4 (R11) pAb at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:Hela whole cell lysate (40ug) Lane4:PMVEC whole cell lysate (40ug) Lane5:AML-12 whole cell lysate (40ug))
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Kv3. 4 (R11) pAb in paraffin-embedded human brain tissue.)
Immunohistochemistry (IHC)
(Immunohistochemistry (IHC) analyzes of Kv3. 4 (R11) pAb in paraffin-embedded human brain tissue.)
Immunoprecipitation (IP)
(Immunoprecipitation of A549 cell lysate using KV3. 4 (R11) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using KV3. 4 (R11) # MBS3004210.“up-arrow” (supernatant) ; “down-arrow (deposition))
Immunoprecipitation (IP)
(Immunoprecipitation of A549 cell lysate using KV3. 4 (R11) pAb (Sepharose Bead Conjugate) (lane 2 and lane 3) and Nonspecific IgG Control (Sepharose Bead Conjugate) (lane 4 ). Lane 1 is 30% input. The Western Blot was probed using KV3. 4 (R11) # MBS3004210.“up-arrow” (supernatant) ; “down-arrow (deposition))
NCBI and Uniprot Product Information
NCBI Description
The Shaker gene family of Drosophila encodes components of voltage-gated potassium channels and is comprised of four subfamilies. Based on sequence similarity, this gene is similar to the Shaw subfamily. The protein encoded by this gene belongs to the delayed rectifier class of channel proteins and is an integral membrane protein that mediates the voltage-dependent potassium ion permeability of excitable membranes. It generates atypical voltage-dependent transient current that may be important for neuronal excitability. Multiple transcript variants have been found for this gene. [provided by RefSeq, Jul 2010]
Uniprot Description
Kv3.4: This protein mediates the voltage-dependent potassium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a potassium-selective channel through which potassium ions may pass in accordance with their electrochemical gradient. Belongs to the potassium channel family. C (Shaw) (TC 1.A.1.2) subfamily. Kv3.4/KCNC4 sub-subfamily. 3 isoforms of the human protein are produced by alternative splicing.
Protein type: Membrane protein, multi-pass; Channel, potassium; Membrane protein, integral
Chromosomal Location of Human Ortholog: 1p21
Cellular Component: voltage-gated potassium channel complex; plasma membrane; integral to membrane; nerve terminal; neuromuscular junction
Molecular Function: voltage-gated potassium channel activity; potassium channel activity; delayed rectifier potassium channel activity
Biological Process: synaptic transmission; regulation of neurotransmitter secretion; protein homooligomerization; potassium ion transport
Research Articles on Kv3.4
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Product Notes
The Kv3.4 kcnc4 (Catalog #AAA3004210) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kv3.4 (R11) Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kv3.4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP). WB: 1:500-1:1000 IHC: 1:50-1:200 IF: 1:50-1:200 IP: 1:10-1:100. Researchers should empirically determine the suitability of the Kv3.4 kcnc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kv3.4, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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