Rabbit anti-Human KSR2 Polyclonal Antibody | anti-KSR2 antibody

KSR2, CT (KSR2, Kinase suppressor of Ras 2) (MaxLight 405)

Cat. Num. AAA6314914

• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: KSR2; Polyclonal Antibody; CT (KSR2; Kinase suppressor of Ras 2) (MaxLight 405); Kinase suppressor of Ras 2; anti-KSR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KSR2.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-KSR2 antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 603-638 in the C-terminal region of human KSR2.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in 293, K562, HepG2, HeLa cell line lysates (35ug/lane) usingMBS6008545. This demonstrates that MBS6008545 detected the KSR2 protein (arrow).)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue using MBS6008545 followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of MBS6008545 for immunohistochemistry.)

Product Categories/Family for anti-KSR2 antibody

Antibodies; Protein Kinases

References

1. Bailey, S.D., et al. Diabetes Care (2010) In press: 2. Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010). 3. Talmud, P.J., et al. Am. J. Hum. Genet. 85(5):628-642(2009). 4. Liu, L., et al. Biochim. Biophys. Acta 1794(10):1485-1495(2009). 5. Zemunik, T., et al. Croat. Med. J. 50(1):23-33(2009).

NCBI and Uniprot Product Information

• NCBI GI #: 167621456
• NCBI GeneID: 283455
• NCBI Accession #: NP_775869.3
• NCBI GenBank Nucleotide #: NM_173598.4
• UniProt Accession #: Q6VAB6; Q3B828; Q8N775; A0PJT2
• Molecular Weight: 107,632 Da
• NCBI Official Full Name: kinase suppressor of Ras 2
• NCBI Official Synonym Full Names: kinase suppressor of ras 2
• NCBI Official Symbol: KSR2
• NCBI Protein Information: kinase suppressor of Ras 2
• UniProt Protein Name: Kinase suppressor of Ras 2
• Protein Family: Kinase suppressor of Ras
• UniProt Gene Name: KSR2
• UniProt Synonym Gene Names: hKSR2
• UniProt Entry Name: KSR2_HUMAN

Uniprot Description

KSR2: Location-regulated scaffold connecting MEK to RAF. Blocks MAP3K8 kinase activity and MAP3K8-mediated signaling. Acts as a negative regulator of MAP3K3-mediated activation of ERK, JNK and NF-kappa-B pathways, inhibiting MAP3K3-mediated interleukin-8 production. Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protein kinase, TKL; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; TKL group; RAF family

Chromosomal Location of Human Ortholog: 12q24.22-q24.23

Cellular Component: membrane; cytoplasm

Molecular Function: protein serine/threonine kinase activity; metal ion binding; ATP binding

Biological Process: protein amino acid phosphorylation