Rabbit anti-Human KRT5 Polyclonal Antibody | anti-KRT5 antibody

KRT5 (Keratin 5, Keratin, Type II Cytoskeletal 5, 58kD Cytokeratin, Cytokeratin-5, CK-5, Keratin-5, K5, Type-II Keratin Kb5) (HRP)

Cat. Num. AAA6383953

• Gene Names: KRT5; K5; CK5; DDD; DDD1; EBS2; KRT5A
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KRT5; Polyclonal Antibody; KRT5 (Keratin 5; Keratin; Type II Cytoskeletal 5; 58kD Cytokeratin; Cytokeratin-5; CK-5; Keratin-5; K5; Type-II Keratin Kb5) (HRP); anti-KRT5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 2247

• Applicable Applications for anti-KRT5 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human KRT5, aa1-590 (AAH24292.1).
• Immunogen Sequence: MSRQSSVSFRSGGSRSFSTASAITPSVSRTSFTSVSRSGGGGGGGFGRVSLAGACGVGGYGSRSLYNLGGSKRISISTSGGSFRNRFGAGAGGGYGFGGGAGSGFGFGGGAGGGFGLGGGAGFGGGFGGPGFPVCPPGGIQEVTVNQSLLTPLNLQIDPSIQRVRTEEREQIKTLNNKFASFIDKVRFLEQQNKVLDTKWTLLQEQGTKTVRQNLEPLFEQYINNLRRQLDSIVGERGRLDSELRNMQDLVEDFKNKYEDEINKRTTAENEFVMLKKDVDAAYMNKVELEAKVDALMDEINFMKMFFDAELSQMQTHVSDTSVVLSMDNNRNLDLDSIIAEVKAQYEEIANRSRTEAESWYQTKYEELQQTAGRHGDDLRNTKHEISEMNRMIQRLRAEIDNVKKQCANLQNAIADAEQRGELALKDARNKLAELEEALQKAKQDMARLLREYQELMNTKLALDVEIATYRKLLEGEECRLSGEGVGPVNISVVTSSVSSGYGSGSGYGGGLGGGLGGGLGGGLAGGGSGSYYSSSSGGVGLSGGLSVGGSGFSASSGRGLGVGFGSGGGSSSSVKFVSTTSSSRKSFKS
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KRT5 rabbit polyclonal antibody. Western Blot analysis of KRT5 expression in HepG2.)

Western Blot (WB)

(Western Blot analysis of KRT5 expression in transfected 293T cell line by KRT5 polyclonal antibody. Lane 1: KRT5 transfected lysate (62.4kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-KRT5 antibody

KRT5 encodes the type II keratin 5, an intermediate filament chain expressed largely in stratified squamous epithelia. Mutations in KRT5 cause epidermolysis bullosa simplex.

Product Categories/Family for anti-KRT5 antibody

Antibodies; Keratin; Cytokeratin

NCBI and Uniprot Product Information

• NCBI GI #: 18999434
• NCBI GeneID: 3852
• UniProt Accession #: P13647
• NCBI Official Full Name: Homo sapiens keratin 5, mRNA
• NCBI Official Synonym Full Names: keratin 5
• NCBI Official Symbol: KRT5
• NCBI Official Synonym Symbols: K5; CK5; DDD; DDD1; EBS2; KRT5A
• NCBI Protein Information: keratin, type II cytoskeletal 5
• UniProt Protein Name: Keratin, type II cytoskeletal 5
• Protein Family: Keratin
• UniProt Gene Name: KRT5
• UniProt Synonym Gene Names: CK-5; K5
• UniProt Entry Name: K2C5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the basal layer of the epidermis with family member KRT14. Mutations in these genes have been associated with a complex of diseases termed epidermolysis bullosa simplex. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]

Uniprot Description

K5: Heterotetramer of two type I and two type II keratins. Keratin-5 associates with keratin-14. Interacts with TCHP. Belongs to the intermediate filament family.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: membrane; mitochondrion; keratin filament; cytoplasm; plasma membrane; intermediate filament; cytosol; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: hemidesmosome assembly; epidermis development

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dowling-degos Disease 1; Epidermolysis Bullosa Simplex With Migratory Circinate Erythema; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Epidermolysis Bullosa Simplex With Mottled Pigmentation; Epidermolysis Bullosa Simplex, Dowling-meara Type