Western Blot (WB)
(Western blot analysis of KRT31/33A/33B expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Rabbit anti-Human, Rat KRT31/33A/33B Polyclonal Antibody | anti-KRT33A antibody
KRT31/33A/33B Antibody
Gene Names
KRT33A; HA3I; K33A; Ha-3I; Krt1-3; hHa3-I; KRTHA3A
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
KRT31/33A/33B; Polyclonal Antibody; KRT31/33A/33B Antibody; Ha-3I; HA3I; Hair keratin; type I Ha3-l; Hard keratin; type I; 3I; hHa3-I; HHA3-l; HKA3A; K33A; Keratin; hair; acidic; 3A; type I cuticular Ha3-I; Keratin-33A; Krt1-3; Krt33a; KRTHA3A; KT33A_HUMAN; type I Ha3-I; HA 3II; HA3II; Hair keratin type I Ha3 II; Hard keratin type I 3II; hHa3 II; hHa3II; HKA3B; K33B; Keratin 33B; Keratin hair acidic 3B; Keratin hair acidic3B; Keratin type I cuticular Ha3 II; Keratin-33B; Keratin33B; KRT 33B; KRT33B; KRTHA 3B; KRTHA3B; KT33B_HUMAN; type I cuticular Ha3-II; type I Ha3-II; Type I hair keratin 3B; Ha-1; HA1; Hair Keratin acidic hHa1; hard keratin; 1; hHa1; HKA1; K1H1_HUMAN; K31; keratin 31; keratin; type I cuticular Ha1; Keratin-31; Krt31; KRTHA1; MGC138630; type I Ha1; anti-KRT33A antibody
Host
Rabbit
Reactivity
Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KRT31/33A/33B antibody detects endogenous levels of total KRT31/33A/33B
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
404
Applicable Applications for anti-KRT33A antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IHC: 1:50-1:200
Immunogen
A synthesized peptide
Tissue Specificity
Expressed in the hair follicles.
Predicted Cross Reactivity
Pig, Bovine, Horse, Dog
Similarity
Pig (90%), Bovine (100%), Horse (100%), Dog (90%)
Conjugation
Unconjugated
Western Blot (WB)
(Western blot analysis of KRT31/33A/33B expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis of KRT31/33A/33B expression in Hela cell lysate, The lane on the left is treated with the antigen-specific peptide.)
Immunohistochemistry (IHC)
(MBS9604067 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Immunohistochemistry (IHC)
(MBS9604067 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
Related Product Information for anti-KRT33A antibody
Function: There are two types of hair/microfibrillar keratin, I (acidic) and II (neutral to basic).
Similarity: Belongs to the intermediate filament family.
Similarity: Belongs to the intermediate filament family.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 47 kDa; 46 kDa; 46 kDa
Predicted: 46 kDa
Predicted: 46 kDa
NCBI Official Full Name
keratin, type I cuticular Ha3-I
NCBI Official Synonym Full Names
keratin 33A
NCBI Official Symbol
KRT33A
NCBI Official Synonym Symbols
HA3I; K33A; Ha-3I; Krt1-3; hHa3-I; KRTHA3A
NCBI Protein Information
keratin, type I cuticular Ha3-I
UniProt Protein Name
Keratin, type I cuticular Ha3-I
Protein Family
UniProt Gene Name
KRT33A
UniProt Synonym Gene Names
HHA3-I; HKA3A; KRTHA3A; K33A
NCBI Description
This gene encodes a member of the keratin gene family. This gene is one of multiple type I hair keratin genes that are clustered in a region of chromosome 17q12-q21 and have the same direction of transcription. As a type I hair keratin, the encoded protein is an acidic protein which heterodimerizes with type II keratins to form hair and nails. There are two isoforms of this protein, encoded by two separate genes, keratin 33A and keratin 33B. [provided by RefSeq, May 2012]
Uniprot Description
MiscellaneousThere are two types of hair/microfibrillar keratin, I (acidic) and II (neutral to basic).
Similar Products
Product Notes
The KRT33A krt33a (Catalog #AAA9604067) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KRT31/33A/33B Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KRT31/33A/33B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). WB: 1:500-1:2000 IHC: 1:50-1:200. Researchers should empirically determine the suitability of the KRT33A krt33a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT31/33A/33B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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