Rabbit anti-Human KRT14 Polyclonal Antibody | anti-KRT14 antibody

KRT14, ID (Keratin, Type I Cytoskeletal 14, Cytokeratin-14, CK-14, Keratin-14, K14) (PE)

Cat. Num. AAA6484238

• Gene Names: KRT14; K14; NFJ; CK14; EBS3; EBS4
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: KRT14; Polyclonal Antibody; ID (Keratin; Type I Cytoskeletal 14; Cytokeratin-14; CK-14; Keratin-14; K14) (PE); anti-KRT14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT14.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KRT14 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:50-1:100; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the center region of human KRT14 (KLH).
• Conjugate: PE
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KRT14 antibody (Center) in mouse NIH-3T3 cell line lysates (35ug/lane). KRT14 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human breast carcinoma with KRT14 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KRT14 Antibody (Center) flow cytometry analysis of MDA-MB231 cells (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KRT14 antibody

Antibodies; Keratin; Cytokeratin

References

1. Barcelos, A.C., J. Cutan. Pathol. 36(6), 647-654 (2009). 2. Romano, R.A., PLoS ONE 4(5), E5623 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 15431310
• NCBI GeneID: 3861
• NCBI Accession #: NP_000517
• NCBI GenBank Nucleotide #: NM_000526
• UniProt Accession #: P02533; Q14715; Q53XY3; Q9BUE3; Q9UBN2; Q9UBN3; Q9UCY4
• Molecular Weight: 51,561 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 14
• NCBI Official Synonym Full Names: keratin 14, type I
• NCBI Official Symbol: KRT14
• NCBI Official Synonym Symbols: K14; NFJ; CK14; EBS3; EBS4
• NCBI Protein Information: keratin, type I cytoskeletal 14; CK-14; cytokeratin 14; cytokeratin-14; keratin 14 (epidermolysis bullosa simplex, Dowling-Meara, Koebner)
• UniProt Protein Name: Keratin, type I cytoskeletal 14
• Protein Family: Keratin
• UniProt Gene Name: KRT14
• UniProt Synonym Gene Names: CK-14; K14
• UniProt Entry Name: K1C14_HUMAN

Uniprot Description

K14: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K5.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21.2

Cellular Component: cytoplasm; keratin filament; intermediate filament; cytosol; nucleus

Molecular Function: protein binding; structural constituent of cytoskeleton

Biological Process: epidermis development; hemidesmosome assembly; epithelial cell differentiation; response to zinc ion; response to ionizing radiation; intermediate filament bundle assembly; hair cycle; aging

Disease: Epidermolysis Bullosa Simplex, Localized; Epidermolysis Bullosa Simplex, Generalized; Dermatopathia Pigmentosa Reticularis; Epidermolysis Bullosa Simplex, Autosomal Recessive 1; Naegeli Syndrome; Epidermolysis Bullosa Simplex, Dowling-meara Type