Western Blot (WB)
(Anti-KRT12 Antibody (C-term)at 1:2000 dilution + mouse eyeball lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Rabbit anti-Human KRT12 Polyclonal Antibody | anti-KRT12 antibody
KRT12 Antibody (C-term)
Western Blot (WB)
(Anti-KRT12 Antibody (C-term)at 1:2000 dilution + mouse eyeball lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-KRT12 Antibody (C-term)at 1:2000 dilution + mouse eyeball lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-KRT12 Antibody (C-term)at 1:500 dilution + Y79 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-KRT12 Antibody (C-term)at 1:500 dilution + Y79 whole cell lysatesLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 53 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(KRT12 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the KRT12 antibody detected the KRT12 protein (arrow).)
Western Blot (WB)
(KRT12 Antibody (C-term) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the KRT12 antibody detected the KRT12 protein (arrow).)
Immunohistochemistry (IHC)
(KRT12 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT12 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Immunohistochemistry (IHC)
(KRT12 Antibdy (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KRT12 Antibdy (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS)
(KRT12 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Flow Cytometry (FC/FACS)
(KRT12 Antibody (C-term) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
keratin 12, expressed in corneal epithelia. Mutations in this gene
lead to Meesmann corneal dystrophy.
Seto, T., et al. Jpn. J. Ophthalmol. 52(3):224-226(2008)
Nielsen, K., et al. Cornea 27(1):100-102(2008)
Sullivan, L.S., et al. Mol. Vis. 13, 975-980 (2007) :
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
NCBI and Uniprot Product Information
NCBI Description
KRT12 encodes the type I intermediate filament chain keratin 12, expressed in corneal epithelia. Mutations in this gene lead to Meesmann corneal dystrophy. [provided by RefSeq, Jul 2008]
Uniprot Description
K12: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity. Defects in KRT12 are a cause of Meesmann corneal dystrophy (MECD); also abbreviated MCD and known as juvenile epithelial corneal dystrophy of Meesmann. MECD is an autosomal dominant disease that causes fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts. Belongs to the intermediate filament family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 17q12
Cellular Component: intermediate filament
Molecular Function: structural molecule activity
Biological Process: visual perception
Disease: Corneal Dystrophy, Meesmann
Research Articles on KRT12
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Product Notes
The KRT12 krt12 (Catalog #AAA9203857) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 442-471. The KRT12 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's KRT12 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS). WB~~1:1000. Researchers should empirically determine the suitability of the KRT12 krt12 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KRT12, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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