Rabbit anti-Human KRT10 Polyclonal Antibody | anti-KRT10 antibody

KRT10, ID (Keratin, Type I Cytoskeletal 10, Cytokeratin-10, CK-10, Keratin-10, K10, KPP) (APC)

Cat. Num. AAA6484207

• Gene Names: KRT10; BIE; EHK; K10; KPP; BCIE; CK10
• Reactivity: Human
• Applications: Immunohistochemistry, Flow Cytometry, Functional Assay, Western Blot, ELISA
• Purity: Purified by Ammonium Sulfate Precipitation.
• Synonyms: KRT10; Polyclonal Antibody; ID (Keratin; Type I Cytoskeletal 10; Cytokeratin-10; CK-10; Keratin-10; K10; KPP) (APC); anti-KRT10 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KRT10.
• Purity/Purification: Purified by Ammonium Sulfate Precipitation.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-KRT10 antibody: Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA)
• Application Notes: IHC: 1:50-1:100; FC/FACS: 1:10-1:50; WB: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide corresponding to a region between corresponding to a portion of amino acids within aa325-354 from the center region of human KRT10, conjugated to KLH.
• Conjugate: APC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: APC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KRT10 antibody (Center) in CEM cell line lysates (35ug/lane). KRT10 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human skin reacted with KRT10 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(KRT10 Antibody (Center) flow cytometric analysis of CEM cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-KRT10 antibody

Antibodies; Keratin; Cytokeratin

References

1. Morais, P., Eur J Dermatol 19(4), 333-336 (2009). 2. Barcelos, A.C., J. Cutan. Pathol. 36(6), 647-654 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 195972866
• NCBI GeneID: 3858
• NCBI Accession #: NP_000412
• NCBI GenBank Nucleotide #: NM_000421
• UniProt Accession #: P13645; Q14664; Q8N175
• Molecular Weight: 58,827 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 10
• NCBI Official Synonym Full Names: keratin 10
• NCBI Official Symbol: KRT10
• NCBI Official Synonym Symbols: BIE; EHK; K10; KPP; BCIE; CK10
• NCBI Protein Information: keratin, type I cytoskeletal 10; CK-10; keratin-10; cytokeratin 10
• UniProt Protein Name: Keratin, type I cytoskeletal 10
• Protein Family: Keratin
• UniProt Gene Name: KRT10
• UniProt Synonym Gene Names: KPP; CK-10; K10
• UniProt Entry Name: K1C10_HUMAN

Uniprot Description

K10: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly. Generally associates with K1.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: extracellular space; membrane; keratin filament; cytoplasm; intermediate filament; nucleus

Molecular Function: structural constituent of epidermis

Biological Process: keratinocyte differentiation

Disease: Erythroderma, Ichthyosiform, Congenital Reticular; Epidermolytic Hyperkeratosis; Ichthyosis, Cyclic, With Epidermolytic Hyperkeratosis