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Western Blot (WB) (Western blot analysis of ACTR3B Antibody (C-term) in K562, CEM, HL-60 cell line lysates (35ug/lane). ACTR3B (arrow) was detected using the purified Pab.)

Rabbit anti-Human, Mouse ACTR3B Polyclonal Antibody | anti-ACTR3B antibody

ACTR3B, CT (Actin-related Protein 3B, Actin-like Protein 3B, ARP3-beta, Actin-related Protein ARP4, ARP4, ARP11) (AP)

Gene Names
ACTR3B; ARP11; ARP3BETA; DKFZp686O24114
Reactivity
Human, Mouse
Applications
Western Blot, ELISA
Purity
Purified by Immunoaffinity chromatography.
Synonyms
ACTR3B; Polyclonal Antibody; CT (Actin-related Protein 3B; Actin-like Protein 3B; ARP3-beta; Actin-related Protein ARP4; ARP4; ARP11) (AP); anti-ACTR3B antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human ACTR3B. Species Crossreactivity: mouse.
Purity/Purification
Purified by Immunoaffinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).
Applicable Applications for anti-ACTR3B antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:100-1:500
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the C-terminal region of human ACTR3B (KLH).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. Do not freeze alkaline phosphatase conjugates which could result in a substantial loss of enzymatic activity. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of ACTR3B Antibody (C-term) in K562, CEM, HL-60 cell line lysates (35ug/lane). ACTR3B (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ACTR3B Antibody (C-term) in K562, CEM, HL-60 cell line lysates (35ug/lane). ACTR3B (arrow) was detected using the purified Pab.)

Western Blot (WB)

(Western blot analysis of ACTR3B Antibody (C-term) in mouse bladder tissue lysates (35ug/lane). ACTR3B (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of ACTR3B Antibody (C-term) in mouse bladder tissue lysates (35ug/lane). ACTR3B (arrow) was detected using the purified Pab.)
Product Categories/Family for anti-ACTR3B antibody
References
1. Ewing, R.M., et.al., Mol. Syst. Biol. 3, 89 (2007). 2. Shindo-Okada, N.et.al., Biochem. Biophys. Res. Commun. 280(1), 61-67 (2001).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,608 Da
NCBI Official Full Name
actin-related protein 3B isoform 1
NCBI Official Synonym Full Names
ARP3 actin-related protein 3 homolog B (yeast)
NCBI Official Symbol
ACTR3B
NCBI Official Synonym Symbols
ARP11; ARP3BETA; DKFZp686O24114
NCBI Protein Information
actin-related protein 3B; ARP3-beta; OTTHUMP00000200055; OTTHUMP00000200076; OTTHUMP00000200077; actin-like protein 3B; actin-related protein ARP4; actin-related protein Arp11; actin-related protein 3-beta
UniProt Protein Name
Actin-related protein 3B
Protein Family
UniProt Gene Name
ACTR3B
UniProt Synonym Gene Names
ARP11; ARP4
UniProt Entry Name
ARP3B_HUMAN

NCBI Description

This gene encodes a member of the actin-related proteins (ARP), which form multiprotein complexes and share 35-55% amino acid identity with conventional actin. The protein encoded by this gene may have a regulatory role in the actin cytoskeleton and induce cell-shape change and motility. Pseudogenes of this gene are located on chromosomes 2, 4, 10, 16, 22 and Y. Alternative splicing results in multiple transcript variants and protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

ACTR3B: Plays a role in the organization of the actin cytoskeleton. May function as ATP-binding component of the Arp2/3 complex which is involved in regulation of actin polymerization and together with an activating nucleation-promoting factor (NPF) mediates the formation of branched actin networks. May decrease the metastatic potential of tumors. Belongs to the actin family. ARP3 subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 7q36.1

Cellular Component: Arp2/3 protein complex; cell projection; cytoplasm

Molecular Function: actin binding; ATP binding

Research Articles on ACTR3B

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Product Notes

The ACTR3B actr3b (Catalog #AAA6464055) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ACTR3B, CT (Actin-related Protein 3B, Actin-like Protein 3B, ARP3-beta, Actin-related Protein ARP4, ARP4, ARP11) (AP) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's ACTR3B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:100-1:500 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the ACTR3B actr3b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ACTR3B, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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