Rabbit KRAS Polyclonal Antibody | anti-KRAS antibody

Anti-KRAS Antibody, Rabbit Polyclonal

Cat. Num. AAA8101437

• Gene Names: KRAS; NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: KRAS; Polyclonal Antibody; Anti-KRAS Antibody; Rabbit Polyclonal; K-Ras Antibody; Rabbit PAb; Antigen Affinity Purified; Kirsten rat sarcoma viral oncogene homolog; Anti-C-K-RAS Antibody; Anti-CFC2 Antibody; Anti-K-RAS Antibody; Anti-K-RAS2A Antibody; Anti-K-RAS2B Antibody; Anti-K-RAS4A Antibody; Anti-K-RAS4B Antibody; Anti-KI-RAS Antibody; Anti-KRAS1 Antibody; Anti-KRAS2 Antibody; Anti-NS Antibody; Anti-NS3 Antibody; Anti-RALD Antibody; Anti-RASK2 Antibody; anti-KRAS antibody

• Host: Rabbit
• Reactivity: Human; Predicted to React with: Mouse, Rat (Species predicted to react based on 100% sequence homology)
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human KRAS
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-KRAS antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human K-Ras
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human K-Ras, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-KRAS rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse lung tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa(We are unsure as to the identity of these extra bands.))

Related Product Information for anti-KRAS antibody

K-Ras belongs to the small GTPase superfamily, Ras family. Like other members of the Ras family, K-Ras is a GTPase and is an early player in many signal transduction pathways. It is usually tethered to cell membranes because of the presence of an isoprenyl group on its C-terminus. K-Ras functions as a molecular on/off switch. Once it is turned on it recruits and activates proteins necessary for the propagation of growth factor and other receptors' signal, such as c-Raf and PI 3-kinase. It binds to GTP in the active state and possesses an intrinsic enzymatic activity that cleaves the terminal phosphate of the nucleotide converting it to GDP. Upon conversion of GTP to GDP, K-Ras is turned off. The rate of conversion is usually slow but can be sped up dramatically by an accessory protein of the GTPase activating protein class, for example, RasGAP. In turn, K-Ras can bind to proteins of the Guanine Nucleotide Exchange Factor class, for example, SOS1, which forces the release of bound nucleotide. Subsequently, K-Ras binds GTP present in the cytosol and the GEF is released from ras-GTP. Besides essential function in normal tissue signaling, the mutation of a K-Ras gene is an essential step in the development of many cancers. Several germline K-Ras mutations are associated with Noonan syndrome and Cardio-Facio-Cutaneous syndrome. Somatic K-Ras mutations are found at high rates in Leukemias, colon cancer, pancreatic cancer, and lung cancer.

Product Categories/Family for anti-KRAS antibody

Cancer Drug Targets; EGFR Signaling Pathway

References

Ling J, et al. (2012) KrasG12D-induced IKK2//NF-B activation by IL-1 alpha and p62 feedforward loops is required for development of pancreatic ductal adenocarcinoma. Cancer Cell. 21(1):105-20.
Matallanas D, et al. (2011) Mutant K-Ras activation of the proapoptotic MST2 pathway is antagonized by wild-type K-Ras. Mol Cell. 44(6):893-906.
Regala RP, et al. (2011) Matrix metalloproteinase-10 promotes Kras-mediated bronchio-alveolar stem cell expansion and lung cancer formation. PLoS One. 6(10):e26439.

NCBI and Uniprot Product Information

• NCBI GI #: 131875
• NCBI GeneID: 3845
• UniProt Accession #: P01116; P01118; Q96D10; A8K8Z5; B0LPF9
• Molecular Weight: 189
• NCBI Official Full Name: GTPase KRas
• NCBI Official Synonym Full Names: Kirsten rat sarcoma viral oncogene homolog
• NCBI Official Symbol: KRAS
• NCBI Official Synonym Symbols: NS; NS3; CFC2; KRAS1; KRAS2; RASK2; KI-RAS; C-K-RAS; K-RAS2A; K-RAS2B; K-RAS4A; K-RAS4B
• NCBI Protein Information: GTPase KRas; K-Ras 2; c-Ki-ras; oncogene KRAS2; K-ras p21 protein; c-Kirsten-ras protein; PR310 c-K-ras oncogene; transforming protein p21; cellular c-Ki-ras2 proto-oncogene; v-Ki-ras2 Kirsten rat sarcoma 2 viral oncogene homolog
• UniProt Protein Name: GTPase KRas
• Protein Family: GTPase
• UniProt Gene Name: KRAS
• UniProt Synonym Gene Names: KRAS2; RASK2
• UniProt Entry Name: RASK_HUMAN

NCBI Description

This gene, a Kirsten ras oncogene homolog from the mammalian ras gene family, encodes a protein that is a member of the small GTPase superfamily. A single amino acid substitution is responsible for an activating mutation. The transforming protein that results is implicated in various malignancies, including lung adenocarcinoma, mucinous adenoma, ductal carcinoma of the pancreas and colorectal carcinoma. Alternative splicing leads to variants encoding two isoforms that differ in the C-terminal region. [provided by RefSeq, Jul 2008]

Uniprot Description

KRas: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity. Interacts with PHLPP. Interacts (active GTP-bound form preferentially) with RGS14. Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase- activating protein (GAP). Belongs to the small GTPase superfamily. Ras family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Oncoprotein; G protein; G protein, monomeric; G protein, monomeric, Ras; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 12p12.1

Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; mitochondrion; membrane; cytoplasm; plasma membrane; cytosol; lipid raft

Molecular Function: protein binding; GDP binding; GTP binding; GMP binding; protein complex binding; LRR domain binding

Biological Process: regulation of long-term neuronal synaptic plasticity; axon guidance; nerve growth factor receptor signaling pathway; activation of MAPKK activity; response to glucocorticoid stimulus; activation of NF-kappaB transcription factor; positive regulation of MAP kinase activity; small GTPase mediated signal transduction; positive regulation of cell proliferation; visual learning; negative regulation of neuron apoptosis; epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; cytokine and chemokine mediated signaling pathway; positive regulation of nitric-oxide synthase activity; MAPKKK cascade; social behavior; response to mineralocorticoid stimulus; regulation of synaptic transmission, GABAergic; positive regulation of Rac protein signal transduction; negative regulation of cell differentiation; Ras protein signal transduction; insulin receptor signaling pathway; innate immune response; striated muscle cell differentiation; positive regulation of protein amino acid phosphorylation; actin cytoskeleton organization and biogenesis; blood coagulation; vascular endothelial growth factor receptor signaling pathway; leukocyte migration

Disease: Bladder Cancer; Gastric Cancer, Hereditary Diffuse; Pancreatic Cancer; Noonan Syndrome 3; Leukemia, Acute Myeloid; Lung Cancer; Breast Cancer; Schimmelpenning-feuerstein-mims Syndrome; Cardiofaciocutaneous Syndrome 2