Rabbit anti-Human, Mouse KPNA5 Polyclonal Antibody | anti-KPNA5 antibody

KPNA5 Antibody

Cat. Num. AAA7046269

• Gene Names: KPNA5; SRP6; IPOA6
• Reactivity: Human, Mouse
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Antigen Affinity Purified
• Synonyms: KPNA5; Polyclonal Antibody; KPNA5 Antibody; Importin subunit alpha-6; Karyopherin subunit alpha-5; anti-KPNA5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Antigen Affinity Purified
• Form/Format: Liquid
• Sequence Length: 536

• Applicable Applications for anti-KPNA5 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Species: Human
• Immunogen: Recombinant human Importin subunit alpha-6 protein (N-240AA)
• Conjugate: Non-conjugated
• Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-68194 / sc-68195
• Preparation and Storage: Shipped at 4 degree C. Upon delivery aliquot and store at -20 degree C or -80 degree C. Avoid repeated freeze.

Western Blot (WB)

(Western blotAll lanes: KPNA5 antibody at 2.04ug/ml+Mouse liver tissueGoat polyclonal to rabbit at 1/10000 dilutionPredicted band size: 60 kDaObserved band size: 60 kDa)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffin-embedded human testis tissue using MBS7046269 at dilution 1:100)

Related Product Information for anti-KPNA5 antibody

Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non-classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.

NCBI and Uniprot Product Information

• NCBI GeneID: 3841
• UniProt Accession #: O15131; Q86X23; B2RAI5
• Molecular Weight: 60,349 Da
• NCBI Official Synonym Full Names: karyopherin subunit alpha 5
• NCBI Official Symbol: KPNA5
• NCBI Official Synonym Symbols: SRP6; IPOA6
• NCBI Protein Information: importin subunit alpha-6
• UniProt Protein Name: Importin subunit alpha-6
• UniProt Gene Name: KPNA5
• UniProt Entry Name: IMA6_HUMAN

NCBI Description

The transport of molecules between the nucleus and the cytoplasm in eukaryotic cells is mediated by the nuclear pore complex (NPC) which consists of 60-100 proteins and is probably 120 million daltons in molecular size. Small molecules (up to 70 kD) can pass through the nuclear pore by nonselective diffusion; larger molecules are transported by an active process. Most nuclear proteins contain short basic amino acid sequences known as nuclear localization signals (NLSs). KPNA5 protein belongs to the importin alpha protein family and is thought to be involved in NLS-dependent protein import into the nucleus. [provided by RefSeq, Jul 2008]

Uniprot Description

KPNA5: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran- dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates nuclear import of STAT1 homodimers and STAT1/STAT2 heterodimers by recognizing non- classical NLSs of STAT1 and STAT2 through ARM repeats 8-9. Recognizes influenza A virus nucleoprotein through ARM repeat 7-9 In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS. Belongs to the importin alpha family.

Protein type: Nuclear import; Karyopherin

Chromosomal Location of Human Ortholog: 6q22.1

Cellular Component: cytoplasm; cytosol; nuclear pore; nucleoplasm

Molecular Function: nuclear localization sequence binding; protein binding; protein transporter activity

Biological Process: modification by virus of host cellular process; NLS-bearing substrate import into nucleus