Rabbit KMT5B Polyclonal Antibody | anti-KMT5B antibody

KMT5B Antibody

Cat. Num. AAA9630240

• Gene Names: SUV420H1; CGI85; KMT5B; CGI-85
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KMT5B; Polyclonal Antibody; KMT5B Antibody; CGI 85; CGI85; Histone-lysine N-methyltransferase KMT5B; Kmt5b; KMT5B_HUMAN; Lysine N-methyltransferase 5B; Lysine-specific methyltransferase 5B; MGC118906; MGC118909; MGC21161; MGC703; Su(var)4-20 homolog 1; Suppressor of variegation 4 20 homolog 1; Suppressor of variegation 4-20 homolog 1; Suv4-20h1; anti-KMT5B antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KMT5B Antibody detects endogenous levels of KMT5B.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KMT5B antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human KMT5B.
• Conjugation: Unconjugated
• Similarity: Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar4-20 subfamily.
• Subunit Structure: Homodimer Ref.14). Interacts with HP1 proteins CBX1, CBX3 and CBX5. Interacts with RB1 family proteins RB1, RBL1 and RBL2 (By similarity). Interacts (via C-terminus) with FRG1.
• Function: Histone methyltransferase that specifically methylates monomethylated 'Lys-20' (H4K20me1) and dimethylated 'Lys-20' (H4K20me2) of histone H4 to produce respectively dimethylated 'Lys-20' (H4K20me2) and trimethylated 'Lys-20' (H4K20me3) and thus regulates transcription and maintenance of genome integrity. In vitro also methylates unmodified 'Lys-20' (H4K20me0) of histone H4 and nucleosomes. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. KMT5B is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2) (By similarity). Plays a role in myogenesis by regulating the expression of target genes, such as EID3. Facilitates TP53BP1 foci formation upon DNA damage and proficient non-homologous end-joining (NHEJ)-directed DNA repair by catalyzing the di- and trimethylation of 'Lys-20' of histone H4. May play a role in class switch reconbination by catalyzing the di- and trimethylation of 'Lys-20' of histone H4 (By similarity).
• Note: Associated with pericentric heterochromatin. CBX1 and CBX5 are required for the localization to pericentric heterochromatin (By similarity).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-KMT5B antibody

Nucleus; Chromosome; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 50659084
• NCBI GeneID: 51111
• NCBI Accession #: NP_057112.3
• NCBI GenBank Nucleotide #: NM_016028.4
• UniProt Accession #: Q4FZB7; Q3SX56; Q4V775; Q6P150; Q96E44; Q9BUL0; Q9H022; Q9H2K3; Q9NXV3; Q9Y393; B7WNX7
• Molecular Weight: 99,187 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase SUV420H1 isoform 2
• NCBI Official Synonym Full Names: suppressor of variegation 4-20 homolog 1 (Drosophila)
• NCBI Official Symbol: SUV420H1
• NCBI Official Synonym Symbols: CGI85; KMT5B; CGI-85
• NCBI Protein Information: histone-lysine N-methyltransferase SUV420H1; suv4-20h1; C630029K18Rik; su(var)4-20 homolog 1; lysine N-methyltransferase 5B
• UniProt Protein Name: Histone-lysine N-methyltransferase SUV420H1
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: SUV420H1
• UniProt Synonym Gene Names: KMT5B; Su(var)4-20 homolog 1; Suv4-20h1
• UniProt Entry Name: SV421_HUMAN

NCBI Description

This gene encodes a protein that contains a SET domain. SET domains appear to be protein-protein interaction domains that mediate interactions with a family of proteins that display similarity with dual-specificity phosphatases (dsPTPases). The function of this gene has not been determined. Two alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

SUV420H1: a protein methyltransferase that specifically trimethylates 'Lys-20' of histone H4. H4 'Lys-20' trimethylation represents a specific tag for epigenetic transcriptional repression. Mainly functions in pericentric heterochromatin regions, thereby playing a central role in the establishment of constitutive heterochromatin in these regions. SUV420H1 is targeted to histone H3 via its interaction with RB1 family proteins (RB1, RBL1 and RBL2). Strongly down-regulated in breast cancer cells. Belongs to the histone-lysine methyltransferase family. Suvar4-20 subfamily. Three alternatively-spliced isoforms have been described.

Protein type: Methyltransferase; Methyltransferase, protein lysine; EC 2.1.1.43; Amino Acid Metabolism - lysine degradation

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: condensed nuclear chromosome, pericentric region

Molecular Function: histone lysine N-methyltransferase activity (H4-K20 specific); histone-lysine N-methyltransferase activity

Biological Process: muscle development; transcription, DNA-dependent; regulation of transcription, DNA-dependent