Rabbit anti-Human KLKB1 Polyclonal Antibody | anti-KLKB1 antibody

KLKB1, CT (KLKB1, KLK3, Plasma kallikrein, Fletcher factor, Kininogenin, Plasma prekallikrein, Plasma kallikrein heavy chain, Plasma kallikrein light chain) (PE)

Cat. Num. AAA6314336

• Gene Names: KLKB1; PPK; KLK3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: KLKB1; Polyclonal Antibody; CT (KLKB1; KLK3; Plasma kallikrein; Fletcher factor; Kininogenin; Plasma prekallikrein; Plasma kallikrein heavy chain; Plasma kallikrein light chain) (PE); Plasma kallikrein light chain; anti-KLKB1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KLKB1.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-KLKB1 antibody: FLISA, Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 508-537 in the C-terminal region of human KLKB1, Swiss/UniProt Accession: P03952.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in K562 cell line lysates (35ug/lane) usingMBS6006203. This demonstrates that MBS6006203 detected the KLKB1 protein (arrow).)

Related Product Information for anti-KLKB1 antibody

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients.

Product Categories/Family for anti-KLKB1 antibody

Antibodies; Kallikrein

References

1. MacKenzie, J.A., et al. Appl Physiol Nutr Metab 35(4):518-525(2010). 2. Han, S., et al. Hum. Immunol. 71(7):727-730(2010). 3. Rajaraman, P., et al. Cancer Epidemiol. Biomarkers Prev. 19(5):1356-1361(2010). 4. Eeckhoudt, S.L., et al. Thromb. Haemost. 103(4):866-867(2010). 5. Barber, M.J., et al. PLoS ONE 5 (3), E9763 (2010).

NCBI and Uniprot Product Information

• NCBI GI #: 78191798
• NCBI GeneID: 3818
• NCBI Accession #: NP_000883.2
• NCBI GenBank Nucleotide #: NM_000892.3
• UniProt Accession #: P03952; Q17RE8; Q17RE9; Q4W5C3; A6NH96; B2R8H9
• Molecular Weight: 71,370 Da
• NCBI Official Full Name: plasma kallikrein preproprotein
• NCBI Official Synonym Full Names: kallikrein B, plasma (Fletcher factor) 1
• NCBI Official Symbol: KLKB1
• NCBI Official Synonym Symbols: PPK; KLK3
• NCBI Protein Information: plasma kallikrein; kininogenin; Fletcher factor; plasma prekallikrein; plasma kallikrein heavy chain; plasma kallikrein light chain
• UniProt Protein Name: Plasma kallikrein
• Protein Family: Plasma kallikrein
• UniProt Gene Name: KLKB1
• UniProt Synonym Gene Names: KLK3
• UniProt Entry Name: KLKB1_HUMAN

NCBI Description

Plasma prekallikrein is a glycoprotein that participates in the surface-dependent activation of blood coagulation, fibrinolysis, kinin generation and inflammation. It is synthesized in the liver and secreted into the blood as a single polypeptide chain. Plasma prekallikrein is converted to plasma kallikrein by factor XIIa by the cleavage of an internal Arg-Ile bond. Plasma kallikrein therefore is composed of a heavy chain and a light chain held together by a disulphide bond. The heavy chain originates from the amino-terminal end of the zymogen and contains 4 tandem repeats of 90 or 91 amino acids. Each repeat harbors a novel structure called the apple domain. The heavy chain is required for the surface-dependent pro-coagulant activity of plasma kallikrein. The light chain contains the active site or catalytic domain of the enzyme and is homologous to the trypsin family of serine proteases. Plasma prekallikrein deficiency causes a prolonged activated partial thromboplastin time in patients. [provided by RefSeq, Jul 2008]

Uniprot Description

KLKB1: The enzyme cleaves Lys-Arg and Arg-Ser bonds. It activates, in a reciprocal reaction, factor XII after its binding to a negatively charged surface. It also releases bradykinin from HMW kininogen and may also play a role in the renin-angiotensin system by converting prorenin into renin. Defects in KLKB1 are the cause of prekallikrein deficiency (PKK deficiency); also known as Fletcher factor deficiency. This disorder is a blood coagulation defect. Belongs to the peptidase S1 family. Plasma kallikrein subfamily.

Protein type: Protease; Secreted; Secreted, signal peptide; EC 3.4.21.34

Chromosomal Location of Human Ortholog: 4q35

Cellular Component: extracellular space; plasma membrane; extracellular region

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: extracellular matrix disassembly; fibrinolysis; extracellular matrix organization and biogenesis; positive regulation of fibrinolysis; zymogen activation; blood coagulation; proteolysis; blood coagulation, intrinsic pathway; Factor XII activation; plasminogen activation

Disease: Prekallikrein Deficiency