Rabbit anti-Human KLK8 Polyclonal Antibody | anti-KLK8 antibody

KLK8, NT (KLK8, NRPN, PRSS19, TADG14, Kallikrein-8, Neuropsin, Ovasin, Serine protease 19, Serine protease TADG-14, Tumor-associated differentially expressed gene 14 protein) (FITC)

Cat. Num. AAA6314307

• Gene Names: KLK8; NP; HNP; NRPN; PRSS19; TADG14
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KLK8; Polyclonal Antibody; NT (KLK8; NRPN; PRSS19; TADG14; Kallikrein-8; Neuropsin; Ovasin; Serine protease 19; Serine protease TADG-14; Tumor-associated differentially expressed gene 14 protein) (FITC); Tumor-associated differentially expressed gene 14 protein; anti-KLK8 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-KLK8 antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:10-50; FLISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KLK8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 8-38 amino acids from the N-terminal region of human KLK8.
• Conjugate: FITC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KLK8 (arrow) using rabbit polyclonal KLK8 Antibody (N-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KLK8 gene.)

Immunohistochemistry (IHC)

(KLK8 Antibody (N-term) (AP14391a)immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KLK8 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-KLK8 antibody

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. Alternate splicing of this gene results in four transcript variants encoding four different isoforms. The isoforms exhibit distinct patterns of expression that suggest roles in brain plasticity and ovarian cancer.

Product Categories/Family for anti-KLK8 antibody

Antibodies; Kallikrein

References

Planque, C., et al. Clin. Chem. 56(6):987-997(2010). Klein, R.J., et al. Cancer Prev Res (Phila) 3(5):611-619(2010). Emami, N., et al. Biol. Chem. 390(9):921-929(2009). Brattsand, M., et al. J. Invest. Dermatol. 129(7):1656-1665(2009). Darling, M.R., et al. Head Neck Pathol 2(3):169-174(2008).

NCBI and Uniprot Product Information

• NCBI GI #: 21464131
• NCBI GeneID: 11202
• NCBI Accession #: NP_653090.1; NP_653088.1
• NCBI GenBank Nucleotide #: NM_144507.2
• UniProt Accession #: O60259; Q5V9X1; Q5V9X2; Q8IW69; Q9HCB3; Q9NR68; Q9NR69; Q9UIL9; Q9UQ47
• Molecular Weight: 3,653 Da
• NCBI Official Full Name: kallikrein-8 isoform 4
• NCBI Official Synonym Full Names: kallikrein-related peptidase 8
• NCBI Official Symbol: KLK8
• NCBI Official Synonym Symbols: NP; HNP; NRPN; PRSS19; TADG14
• NCBI Protein Information: kallikrein-8; ovasin; serine protease 19; serine protease TADG-14; tumor-associated differentially expressed gene 14 protein
• UniProt Protein Name: Kallikrein-8
• Protein Family: Kallikrein
• UniProt Gene Name: KLK8
• UniProt Synonym Gene Names: NRPN; PRSS19; TADG14; hK8; NP
• UniProt Entry Name: KLK8_HUMAN

Uniprot Description

KLK8: Serine protease which is capable of degrading a number of proteins such as casein, fibrinogen, kininogen, fibronectin and collagen type IV. Also cleaves L1CAM in response to increased neural activity. Induces neurite outgrowth and fasciculation of cultured hippocampal neurons. Plays a role in the formation and maturation of orphan and small synaptic boutons in the Schaffer- collateral pathway, regulates Schaffer-collateral long-term potentiation in the hippocampus and is required for memory acquisition and synaptic plasticity. Involved in skin desquamation and keratinocyte proliferation. Plays a role in the secondary phase of pathogenesis following spinal cord injury. Belongs to the peptidase S1 family. Kallikrein subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; EC 3.4.21.118; Protease; Secreted; Cell development/differentiation

Chromosomal Location of Human Ortholog: 19q13

Cellular Component: extracellular space; cytoplasm

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: cell death; negative regulation of myelination; negative regulation of axon regeneration; synapse organization and biogenesis; response to wounding; regulation of synapse organization and biogenesis; keratinocyte proliferation; proteolysis; neurite morphogenesis; memory