Western Blot (WB)
(Western Blot analysis of extracts of various cells, using KLK10 antibody.)
Rabbit KLK10 Polyclonal Antibody | anti-KLK10 antibody
KLK10 Polyclonal Antibody
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunohistochemistry, Immunofluorescence
Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Synonyms
KLK10; Polyclonal Antibody; KLK10 Polyclonal Antibody; Kallikrein-10; Normal epithelial cell-specific 1; Protease serine-like 1; NES1; PRSSL1; anti-KLK10 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
KLK10 polyclonal antibody detects endogenous levels of KLK10 protein.
Purity/Purification
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
Form/Format
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Sequence Length
276
Applicable Applications for anti-KLK10 antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)
Application Notes
WB: 1:500-1:2000
IHC: 1:50-1:200
IF: 1:50-1:200
IHC: 1:50-1:200
IF: 1:50-1:200
Immunogen
Recombinant full length Human KLK10.
Preparation and Storage
Store at 4 degree C short term.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Aliquot and store at -20 degree C long term.
Avoid freeze-thaw cycles.
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using KLK10 antibody.)
Western Blot (WB)
(Western Blot analysis of extracts of various cells, using KLK10 antibody.)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using KLK10 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human liver cancer using KLK10 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using KLK10 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC)
(Immunohistochemistry of paraffin-embedded human prostate using KLK10 antibody at dilution of 1:100 (40x lens).)
Related Product Information for anti-KLK10 antibody
Kallikreins (KLKs) belong to the serine protease family of proteolytic enzymes. Human pancreatic/renal KLK encodes for the KLK1 enzyme, which is involved in post-translational processing of polypeptide precursors. The function of the other members of KLK gene family is currently unknown, but evidence suggests that many KLKs are implicated in carcinogenesis. The human KLK gene family consists of 15 serine proteases. The human KLK genes are clustered on chromosome 19q13. Unlike other kalllikreins, the KLK4-15 encoded proteases are less related and do not contain a conventional KLK loop. Clusters of genes exhibit high prostatic (KLK2-4, KLK15) or pancreatic (KLK6-13) expression. KLK2 is also known as glandular kallikrein 2, tissue kallikrein or HGK-1, and KLK3 is known as prostate-specific antigen (PSA). Both KLK2 and KLK3 have important applications in prostate cancer and breast cancer tests. KLK4, KLK5, KLK9, KLK13, KLK12 and KLK14 have been previously known as KLK-L1, KLK-L2, KLK-L3, KLK-L4, KLK-L5 and KLK-L6, respectively. Many of the KLKs are regulated by steroid hormones and a few of them, specifically KLK3, KLK6 and KLK10, are known to be downregulated in breast and other cancers.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
~ 30kDa
NCBI Official Full Name
kallikrein-10 preproprotein
UniProt Protein Name
Kallikrein-10
Protein Family
UniProt Gene Name
KLK10
UniProt Synonym Gene Names
NES1; PRSSL1
UniProt Entry Name
KLK10_HUMAN
Uniprot Description
KLK10: Has a tumor-suppressor role for NES1 in breast and prostate cancer. Belongs to the peptidase S1 family. Kallikrein subfamily.
Protein type: Tumor suppressor; Secreted; Secreted, signal peptide; EC 3.4.21.-; Protease
Chromosomal Location of Human Ortholog: 19q13
Cellular Component: extracellular region
Molecular Function: serine-type peptidase activity; serine-type endopeptidase activity
Biological Process: proteolysis; cell cycle
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Product Notes
The KLK10 klk10 (Catalog #AAA3011547) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KLK10 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KLK10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF). WB: 1:500-1:2000 IHC: 1:50-1:200 IF: 1:50-1:200. Researchers should empirically determine the suitability of the KLK10 klk10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KLK10, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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