Western Blot (WB)
(Western blot analysis on HeLa cell lysate using ABCF1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Rabbit ABCF1 Polyclonal Antibody | anti-ABCF1 antibody
ABCF1 Antibody
Gene Names
ABCF1; ABC27; ABC50
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunofluorescence, Immunocytochemistry, ELISA
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Synonyms
ABCF1; Polyclonal Antibody; ABCF1 Antibody; ABC27; ABC50; ABCF1_HUMAN; ATP binding cassette 50 (TNF alpha stimulated); ATP binding cassette 50; ATP binding cassette sub family F member 1; ATP binding cassette; sub family F (GCN20); member 1; ATP-binding cassette 50; ATP-binding cassette sub-family F member 1; AU041969; D17Wsu166e; EST123147; GCN20; TNF alpha stimulated ABC protein; TNF-alpha-stimulated ABC protein; TNFalpha inducible ATP binding protein; wu:fb79c06; wu:fc39a05; wu:fj94a08; zgc:85667; anti-ABCF1 antibody
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
ABCF1 antibody detects endogenous levels of total ABCF1
Purity/Purification
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Form/Format
Liquid
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
845
Applicable Applications for anti-ABCF1 antibody
Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA)
Application Notes
WB: 1:500-1:3000
IF/ICC: 1:100-1:500
IF/ICC: 1:100-1:500
Immunogen
A synthesized peptide derived from human ABCF1
Subcellular Location
Cytoplasm. Nucleus, Nucleoplasm. Nucleus Envelope.
Tissue Specificity
Ubiquitous.
Predicted Cross Reactivity
Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
Similarity
Pig (91%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Xenopus (91%)
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis on HeLa cell lysate using ABCF1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Western Blot (WB)
(Western blot analysis on HeLa cell lysate using ABCF1 Antibody, The lane on the left is treated with the antigen-specific peptide.)
Immunofluorescene (IF)
(MBS9600313 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Immunofluorescene (IF)
(MBS9600313 staining HeLa by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
Related Product Information for anti-ABCF1 antibody
Description: ABCF1 May function in mRNA translation. Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily. Induced by TNF (in cultured synoviocytes). Interacts with eukaryotic initiation factor 2 (eIF2). Associates with the ribosomes. 2 isoforms of the human protein are produced by alternative splicing. Note: This description may include information from UniProtKB.
Function: Isoform 2 is required for efficient Cap-and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Subunit Structure: Isoform 2 interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Isoform 2 associates (via both ABC transporter domains) with the ribosomes.
Post-translational Modifications: Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.
Similarity: Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
Function: Isoform 2 is required for efficient Cap-and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Subunit Structure: Isoform 2 interacts (via N-terminus) with EIF2S1; the interaction is independent of its phosphorylated status. Isoform 2 associates (via both ABC transporter domains) with the ribosomes.
Post-translational Modifications: Isoform 2 is phosphorylated at phosphoserine and phosphothreonine. Isoform 2 phosphorylation on Ser-109 and Ser-140 by CK2 inhibits association of EIF2 with ribosomes.
Similarity: Belongs to the ABC transporter superfamily. ABCF family. EF3 subfamily.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
Observed: 95 kDa
Predicted: 96 kDa
Predicted: 96 kDa
NCBI Official Full Name
ATP-binding cassette sub-family F member 1 isoform a
NCBI Official Synonym Full Names
ATP binding cassette subfamily F member 1
NCBI Official Symbol
ABCF1
NCBI Official Synonym Symbols
ABC27; ABC50
NCBI Protein Information
ATP-binding cassette sub-family F member 1
UniProt Protein Name
ATP-binding cassette sub-family F member 1
Protein Family
UniProt Gene Name
ABCF1
UniProt Synonym Gene Names
ABC50
NCBI Description
The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the GCN20 subfamily. Unlike other members of the superfamily, this protein lacks the transmembrane domains which are characteristic of most ABC transporters. This protein may be regulated by tumor necrosis factor-alpha and play a role in enhancement of protein synthesis and the inflammation process. [provided by RefSeq, Jul 2008]
Uniprot Description
Isoform 2 is required for efficient Cap- and IRES-mediated mRNA translation initiation. Isoform 2 is not involved in the ribosome biogenesis.
Research Articles on ABCF1
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Product Notes
The ABCF1 abcf1 (Catalog #AAA9600313) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The ABCF1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's ABCF1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC), ELISA (EIA). WB: 1:500-1:3000 IF/ICC: 1:100-1:500. Researchers should empirically determine the suitability of the ABCF1 abcf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ABCF1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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