Rabbit KLHL22 Polyclonal Antibody | anti-KLHL22 antibody

KLHL22 Antibody

Cat. Num. AAA9629059

• Gene Names: KLHL22; KELCHL
• Reactivity: Human, Mouse, Rat
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KLHL22; Polyclonal Antibody; KLHL22 Antibody; 2610318I18Rik; kelch-like 22; kelch-like family member 22; Kelch-like protein 22; KELCHL; KLH22_HUMAN; Klhl22; RGD1306288; anti-KLHL22 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KLHL22 Antibody detects endogenous levels of total KLHL22.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KLHL22 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Subunit Structure: Component of the BCR(KLHL22) E3 ubiquitin ligase complex, at least composed of CUL3, KLHL22 and RBX1. Interacts with PLK1. Interacts with DEPDC5 (via DEP domain); the interaction depends on amino acid availability. Interacts with YWHAE; required for the nuclear localization of KLHL22 upon amino acid starvation.
• Function: Substrate-specific adapter of a BCR (BTB-CUL3-RBX1) E3 ubiquitin ligase complex required for chromosome alignment and localization of PLK1 at kinetochores. The BCR(KLHL22) ubiquitin ligase complex mediates monoubiquitination of PLK1, leading to PLK1 dissociation from phosphoreceptor proteins and subsequent removal from kinetochores, allowing silencing of the spindle assembly checkpoint (SAC) and chromosome segregation. Monoubiquitination of PLK1 does not lead to PLK1 degradation. The BCR(KLHL22) ubiquitin ligase complex is also responsible for the amino acid-stimulated 'Lys-48' polyubiquitination and proteasomal degradation of DEPDC5. Through the degradation of DEPDC5, releases the GATOR1 complex-mediated inhibition of the TORC1 pathway. It is therefore an amino acid-dependent activator within the amino acid-sensing branch of the TORC1 pathway, indirectly regulating different cellular processes including cell growth and autophagy.
• Note: Mainly cytoplasmic in prophase and prometaphase. Associates with the mitotic spindle as the cells reach chromosome bi-orientation. Localizes to the centrosomes shortly before cells enter anaphase After anaphase onset, predominantly associates with the polar microtubules connecting the 2 opposing centrosomes and gradually diffuses into the cytoplasm during telophase (PubMed:23455478). Localizes to the nucleus upon amino acid starvation (PubMed:29769719). Relocalizes to the cytosol and associates with lysosomes when amino acids are available (PubMed:29769719).
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-KLHL22 antibody

Cytoplasm; Cytosol; Cytoskeleton; Microtubule organizing center; Centrosome; Spindle; Nucleus; Lysosome; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 24432026
• NCBI GeneID: 84861
• NCBI Accession #: NP_116164
• NCBI GenBank Nucleotide #: NM_032775
• UniProt Accession #: Q53GT1; Q96B68; Q96KC6; A8K3Q4; A8MTV3; B7Z2G1; D3DX30
• Molecular Weight: 55,567 Da
• NCBI Official Full Name: kelch-like protein 22
• NCBI Official Synonym Full Names: kelch-like family member 22
• NCBI Official Symbol: KLHL22
• NCBI Official Synonym Symbols: KELCHL
• NCBI Protein Information: kelch-like protein 22; kelch-like 22
• UniProt Protein Name: Kelch-like protein 22
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL22
• UniProt Entry Name: KLH22_HUMAN