Rabbit KLHL15 Polyclonal Antibody | anti-KLHL15 antibody

Rabbit Polyclonal to Human KLHL15

Cat. Num. AAA247763

• Gene Names: KLHL15; HEL-S-305
• Reactivity: Human, Mouse, Rat
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: KLHL15; Polyclonal Antibody; Rabbit Polyclonal to Human KLHL15; Anti-KLHL15 Antibody (C-Terminus) IHC-plus; Kelch-like 15 (Drosophila); KIAA1677; Kelch-like protein 15; Human KLHL15; anti-KLHL15 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: Human KLHL15
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.02% sodium azide
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 604

• Applicable Applications for anti-KLHL15 antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, ICC, IHC-P (5 ug/ml), WB (1 - 2 ug/ml); Usage: KLHL15 antibody can be used for detection of KLHL15 by Western blot at 1-2 ug/ml.
• Target Species: Human
• Immunogen Description: A 15 amino acid peptide near the carboxy terminus of human KLHL15.
• Immunogen Type: Synthetic peptide
• Immunogen: KLHL15 antibody was raised against a 15 amino acid peptide near the carboxy terminus of human KLHL15.
• Antigen Modification: C-Terminus
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles. Store undiluted.

Immunohistochemistry (IHC)

(Anti-KLHL15 antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of KLHL15 in HeLa cells with KLHL15 antibody at 20 ug/ml.)

Immunocytochemistry (ICC)

(Immunocytochemistry of KLHL15 in HeLa cells with KLHL15 antibody at 5 ug/ml.)

Western Blot (WB)

(Western blot of KLHL15 in HeLa cell lysate with KLHL15 antibody at (A) 1 and (B) 2 ug/ml.)

NCBI and Uniprot Product Information

• NCBI GI #: 226442729
• NCBI GeneID: 80311
• NCBI Accession #: NP_085127.2
• NCBI GenBank Nucleotide #: NM_030624.2
• UniProt Accession #: Q96M94; Q32MN3; Q8NDA3; Q96BM6; Q9C0I6
• Molecular Weight: 69,775 Da
• NCBI Official Full Name: kelch-like protein 15
• NCBI Official Synonym Full Names: kelch-like family member 15
• NCBI Official Symbol: KLHL15
• NCBI Official Synonym Symbols: HEL-S-305
• NCBI Protein Information: kelch-like protein 15; kelch-like 15; epididymis secretory protein Li 305
• UniProt Protein Name: Kelch-like protein 15
• Protein Family: Kelch-like protein
• UniProt Gene Name: KLHL15
• UniProt Synonym Gene Names: KIAA1677
• UniProt Entry Name: KLH15_HUMAN

NCBI Description

This gene encodes a member of the kelch-like family of proteins that share a common domain structure consisting of an N-terminal broad-complex, tramtrack, bric-a-brac/poxvirus and zinc finger domain and C-terminal kelch repeat motifs. The encoded protein may be involved in protein ubiquitination and cytoskeletal organization. [provided by RefSeq, Apr 2009]

Uniprot Description

KLHL15: a substrate-specific adapter for the recruitment of substrates to Cul3-based E3 ubiquitin ligases. KLHL15-Cul3 specifically targets the PP2A regulatory subunit PPP2R5B to promote its turnover by ubiquitylation and proteasomal degradation. May contribute to the selective expression of the PP2A/B holoenzyme in the brain.

Protein type: Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: Xp22.1-p21

Biological Process: protein ubiquitination