Rabbit anti-Human KLF17 Polyclonal Antibody | anti-KLF17 antibody

KLF17 Antibody (C-term)

Cat. Num. AAA9200974

• Gene Names: KLF17; ZNF393; Zfp393
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry, Functional Assay
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: KLF17; Polyclonal Antibody; KLF17 Antibody (C-term); Krueppel-like factor 17; Zinc finger protein 393; ZNF393; anti-KLF17 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This KLF17 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 243-271 amino acids from the C-terminal region of human KLF17.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Vial Concentration: 0.5 (varies by lot)
• Sequence Positions: 243-271
• Sequence Length: 389

• Applicable Applications for anti-KLF17 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC/FACS)
• Application Notes: WB~~1:1000
• Antigen Type: Synthetic Peptide
• Antigen Source: HUMAN
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(KLF17 Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the KLF17 antibody detected the KLF17 protein (arrow).)

Flow Cytometry (FC/FACS)

(KLF17 Antibody (C-term) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KLF17 antibody

KLF17 binds G/C-rich sites via its zinc fingers and activates transcription from CACCC-box elements. It may be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development.

References

Gumireddy, K., et al. Nat. Cell Biol. 11(11):1297-1304(2009) van Vliet, J., et al. Genomics 87(4):474-482(2006) Yan, W., et al. Mech. Dev. 118 (1-2), 233-239 (2002)

NCBI and Uniprot Product Information

• NCBI GI #: 104294874
• NCBI GeneID: 128209
• NCBI Accession #: NP_775755.3
• NCBI GenBank Nucleotide #: NM_173484.3
• UniProt Accession #: Q5JT82; Q86VQ7; Q8N805
• Molecular Weight: 42577
• NCBI Official Full Name: Krueppel-like factor 17
• NCBI Official Synonym Full Names: Kruppel-like factor 17
• NCBI Official Symbol: KLF17
• NCBI Official Synonym Symbols: ZNF393; Zfp393
• NCBI Protein Information: Krueppel-like factor 17
• UniProt Protein Name: Krueppel-like factor 17
• Protein Family: Krueppel-like factor
• UniProt Gene Name: KLF17
• UniProt Synonym Gene Names: ZNF393
• UniProt Entry Name: KLF17_HUMAN

Uniprot Description

KLF17: Transcription repressor that binds to the promoter of target genes and prevents their expression. Acts as a negative regulator of epithelial-mesenchymal transition and metastasis in breast cancer. Specifically binds the 5'-CACCC-3' sequence in the promoter of ID1, a key metastasis regulator in breast cancer, and repress its expression. May be a germ cell-specific transcription factor that plays important roles in spermatid differentiation and oocyte development. Belongs to the Sp1 C2H2-type zinc-finger protein family.

Protein type: C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 1p34.1

Cellular Component: nucleus

Molecular Function: metal ion binding; transcription factor activity

Biological Process: regulation of transcription from RNA polymerase II promoter; transcription, DNA-dependent; gamete generation