Western Blot (WB)
(Western blot analysis of Kizuna (Phospho-Thr379) using UV treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Rabbit Kizuna Polyclonal Antibody | anti-Kizuna antibody
Phospho-Kizuna (Thr379) Antibody
Gene Names
KIZ; RP69; HT013; Kizuna; PLK1S1; C20orf19; NCRNA00153
Reactivity
HumanPredicted Reactivity: Pig (88%), Xenopus (88%)
Applications
Western Blot, ELISA
Purity
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Synonyms
Kizuna; Polyclonal Antibody; Phospho-Kizuna (Thr379) Antibody; Centrosomal protein kizuna; DKFZP586H021; HT013; Kiz; KIZ_HUMAN; KIZUNA; MGC102941; MGC141930; NCRNA00153; Plk1s1; Polo-like kinase 1 substrate 1; Uncharacterized protein C20orf19; anti-Kizuna antibody
Host
Rabbit
Reactivity
Human
Predicted Reactivity: Pig (88%), Xenopus (88%)
Predicted Reactivity: Pig (88%), Xenopus (88%)
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Phospho-Kizuna (Thr379) Antibody detects endogenous levels of Kizuna only when phosphorylated at Thr379.
Purity/Purification
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
Form/Format
Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-Kizuna antibody
Western Blot (WB), Peptide ELISA (EIA)
Application Notes
WB: 1:1000-3000
Peptide ELISA: 1:20,000-1:40,000
Peptide ELISA: 1:20,000-1:40,000
Immunogen
A synthesized peptide derived from human Kizuna around the phosphorylation site of Thr379.
Conjugation
Unconjugated
Fragment
Fab fragment
Post Translational Modifications
Phosphorylation at Thr-379 by PLK1 is not needed for centrosomal localization or pericentriolar material expansion but is indispensable for spindle-pole stabilization.
Subunit Structure
Interacts with AKAP9, CEP72, ODF2, PCNT and TUBGCP2.
Similarity
Belongs to the kizuna family.
Subcellular Location
Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.
Note: Localizes to centrosomes throughout the cell cycle. After centrosome duplication, it usually remains associated only with the mother centrosome, containing the older mature centriole and particles surrounding it. During prophase, additional particles accumulate around both separating centrosomes. Does not accumulate at the microtubule minus ends, but instead localizes to the centrosomes and centrosome-surrounding area in a microtubule-independent and dependent manner, respectively.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Western blot analysis of Kizuna (Phospho-Thr379) using UV treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Western Blot (WB)
(Western blot analysis of Kizuna (Phospho-Thr379) using UV treated 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)
Related Product Information for anti-Kizuna antibody
Centrosomal protein required for establishing a robust mitotic centrosome architecture that can endure the forces that converge on the centrosomes during spindle formation. Required for stabilizing the expanded pericentriolar material around the centriole.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
59,243 Da
NCBI Official Full Name
centrosomal protein kizuna isoform 2
NCBI Official Synonym Full Names
kizuna centrosomal protein
NCBI Official Symbol
KIZ
NCBI Official Synonym Symbols
RP69; HT013; Kizuna; PLK1S1; C20orf19; NCRNA00153
NCBI Protein Information
centrosomal protein kizuna; polo-like kinase 1 substrate 1
UniProt Protein Name
Centrosomal protein kizuna
UniProt Gene Name
KIZ
UniProt Synonym Gene Names
C20orf19; NCRNA00153; PLK1S1
UniProt Entry Name
KIZ_HUMAN
Similar Products
Product Notes
The Kizuna kiz (Catalog #AAA9614278) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-Kizuna (Thr379) Antibody reacts with Human Predicted Reactivity: Pig (88%), Xenopus (88%) and may cross-react with other species as described in the data sheet. AAA Biotech's Kizuna can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Peptide ELISA (EIA). WB: 1:1000-3000 Peptide ELISA: 1:20,000-1:40,000. Researchers should empirically determine the suitability of the Kizuna kiz for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Kizuna, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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