Rabbit KISS1R / GPR54 Polyclonal Antibody | anti-KISS1R antibody

Rabbit Polyclonal to Human KISS1R / GPR54

Cat. Num. AAA244364

• Gene Names: KISS1R; HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
• Reactivity: Human; Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: KISS1R / GPR54; Polyclonal Antibody; Rabbit Polyclonal to Human KISS1R / GPR54; Anti-KISS1R / GPR54 Antibody (C-Terminus) IHC-plus; KISS1R; AXOR12; GPR54; HOT7T175; KISS-1R; KISS1 receptor; G protein-coupled receptor 54; Metastin receptor; Rot7t175; G-protein coupled receptor 54; HH8; Hypogonadotropin-1; KiSS-1 receptor; Kisspeptins receptor; Human KISS1R; anti-KISS1R antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Gibbon, Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human KISS1R / GPR54. BLAST analysis of the peptide immunogen showed no homology with other human proteins, except ZC3H3 (50%).
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 398

• Applicable Applications for anti-KISS1R antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (3 - 5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 20 amino acid peptide from C-terminus of human KISS1R / GPR54. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (95%); Marmoset (80%).
• Immunogen Type: Synthetic peptide
• Immunogen: KISS1R / GPR54 antibody was raised against synthetic 20 amino acid peptide from C-terminus of human KISS1R / GPR54. Percent identity with other species by BLAST analysis: Human, Gibbon, Monkey (95%); Marmoset (80%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Long term: -70 degree C; Short term: +4 degree C

Immunohistochemistry (IHC)

(Anti-KISS1R / GPR54 antibody IHC of human brain, cerebellum. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3-5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-KISS1R / GPR54 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 3-5 ug/ml.)

Related Product Information for anti-KISS1R antibody

GPR54 is a Metastin Receptor. Metastin, also known as KiSS-1, is a metastasis suppressor gene. Mutations in GPR54 have been shown to cause autosomal recessive idiopathic hypogonadotropic hypogonadism in humans and mice, suggesting that GPR54 is a key regulator for the onset of puberty (Seminara et al. 2003).

Product Categories/Family for anti-KISS1R antibody

Family: GPCR; Subfamily: Metastin

NCBI and Uniprot Product Information

• NCBI GI #: 62865887
• NCBI GeneID: 84634
• NCBI Accession #: NP_115940.2
• NCBI GenBank Nucleotide #: NM_032551.4
• UniProt Accession #: Q969F8; Q96QG0; A5D8U2; B2RTV1
• Molecular Weight: 42,586 Da
• NCBI Official Full Name: kiSS-1 receptor
• NCBI Official Synonym Full Names: KISS1 receptor
• NCBI Official Symbol: KISS1R
• NCBI Official Synonym Symbols: HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
• NCBI Protein Information: kiSS-1 receptor; metastin receptor; hypogonadotropin-1; kisspeptins receptor; G protein-coupled receptor 54; G-protein coupled receptor OT7T175
• UniProt Protein Name: KiSS-1 receptor
• Protein Family: KiSS-1 receptor
• UniProt Gene Name: KISS1R
• UniProt Synonym Gene Names: AXOR12; GPR54; KiSS-1R; hOT7T175
• UniProt Entry Name: KISSR_HUMAN

NCBI Description

The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty. [provided by RefSeq, Jul 2008]

Uniprot Description

KISS1R: Receptor for metastin (kisspeptin-54 or kp-54), a C- terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine- tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins. Defects in KISS1R are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Defects in KISS1R are a cause of central precocious puberty (CEPREPU). Precocious puberty is defined as the development of secondary sexual characteristics before the age of 8 years in girls and 9 years in boys. Central precocious puberty refers to a gonadotropin-dependent type which results from premature activation of the hypothalamic-pituitary-gonadal axis. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell cycle regulation; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell surface; integral to membrane; plasma membrane

Molecular Function: neuropeptide binding; neuropeptide receptor activity; protein binding

Biological Process: negative regulation of cell proliferation; G-protein coupled receptor protein signaling pathway; activation of MAPKK activity; neuropeptide signaling pathway; positive regulation of stress fiber formation; calcium-mediated signaling; positive regulation of hormone secretion; arachidonic acid secretion; positive regulation of synaptic transmission

Disease: Hypogonadotropic Hypogonadism 8 With Or Without Anosmia; Precocious Puberty, Central, 1