Human KiSS-1 receptor ELISA Kit | KISS1R elisa kit

Human KiSS-1 receptor ELISA Kit

Cat. Num. AAA764412

• Gene Names: KISS1R; HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
• Reactivity: Human
• Synonyms: KiSS-1 receptor; Human KiSS-1 receptor ELISA Kit; KISS1R/HH8/GPR54/AXOR12/KISS-1R/HOT7T175/Metastin receptor/Kisspeptins receptor/Hypogonadotropin-1/G-protein coupled receptor 54/G-protein coupled receptor OT7T175 (hOT7T175)/AXOR12/GPR54//OT7T175/AXOR12hypogonadotropin-1/GPR54kiSS-1R/KISS1 recepto; KISS1R elisa kit

• Reactivity: Human
• Sequence Length: 398

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 1.56-100ng/ml
• Sensitivity: 0.938ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for KISS1R elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Human KISS1R antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human KISS1R antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human KISS1R amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human KISS1R can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 62865887
• NCBI GeneID: 84634
• NCBI Accession #: NP_115940.2
• NCBI GenBank Nucleotide #: NM_032551.4
• Molecular Weight: 42,586 Da
• NCBI Official Full Name: kiSS-1 receptor
• NCBI Official Synonym Full Names: KISS1 receptor
• NCBI Official Symbol: KISS1R
• NCBI Official Synonym Symbols: HH8; CPPB1; GPR54; AXOR12; KISS-1R; HOT7T175
• NCBI Protein Information: kiSS-1 receptor
• UniProt Protein Name: KiSS-1 receptor
• Protein Family: KiSS-1 receptor
• UniProt Gene Name: KISS1R
• UniProt Synonym Gene Names: AXOR12; GPR54; KiSS-1R; hOT7T175
• UniProt Entry Name: KISSR_HUMAN

NCBI Description

The protein encoded by this gene is a galanin-like G protein-coupled receptor that binds metastin, a peptide encoded by the metastasis suppressor gene KISS1. The tissue distribution of the expressed gene suggests that it is involved in the regulation of endocrine function, and this is supported by the finding that this gene appears to play a role in the onset of puberty. Mutations in this gene have been associated with hypogonadotropic hypogonadism and central precocious puberty. [provided by RefSeq, Jul 2008]

Uniprot Description

KISS1R: Receptor for metastin (kisspeptin-54 or kp-54), a C- terminally amidated peptide of KiSS1. KiSS1 is a metastasis suppressor protein that suppresses metastases in malignant melanomas and in some breast carcinomas without affecting tumorigenicity. The metastasis suppressor properties may be mediated in part by cell cycle arrest and induction of apoptosis in malignant cells. The receptor is essential for normal gonadotropin-released hormone physiology and for puberty. The hypothalamic KiSS1/KISS1R system is a pivotal factor in central regulation of the gonadotropic axis at puberty and in adulthood. The receptor is also probably involved in the regulation and fine- tuning of trophoblast invasion generated by the trophoblast itself. Analysis of the transduction pathways activated by the receptor identifies coupling to phospholipase C and intracellular calcium release through pertussis toxin-insensitive G(q) proteins. Defects in KISS1R are a cause of idiopathic hypogonadotropic hypogonadism (IHH). IHH is defined as a deficiency of the pituitary secretion of follicle-stimulating hormone and luteinizing hormone, which results in the impairment of pubertal maturation and of reproductive function. Defects in KISS1R are a cause of central precocious puberty (CEPREPU). Precocious puberty is defined as the development of secondary sexual characteristics before the age of 8 years in girls and 9 years in boys. Central precocious puberty refers to a gonadotropin-dependent type which results from premature activation of the hypothalamic-pituitary-gonadal axis. Belongs to the G-protein coupled receptor 1 family.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Cell cycle regulation; Receptor, GPCR; GPCR, family 1

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: cell surface; integral to membrane; integral to plasma membrane; intracellular membrane-bound organelle; plasma membrane

Molecular Function: neuropeptide binding; neuropeptide receptor activity; protein binding

Biological Process: activation of MAPKK activity; arachidonic acid secretion; calcium-mediated signaling; G-protein coupled receptor protein signaling pathway; G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); negative regulation of cell proliferation; neuropeptide signaling pathway; positive regulation of hormone secretion; positive regulation of stress fiber formation; positive regulation of synaptic transmission

Disease: Hypogonadotropic Hypogonadism 8 With Or Without Anosmia; Precocious Puberty, Central, 1