Rabbit Kinesin 5 C Polyclonal Antibody | anti-KIF5C antibody

Kinesin 5 C Antibody

Cat. Num. AAA9612315

• Gene Names: KIF5C; KINN; NKHC; NKHC2; CDCBM2; NKHC-2
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: Kinesin 5 C; Polyclonal Antibody; Kinesin 5 C Antibody; CDCBM2; FLJ44735; KIAA0531; KIF 5C; Kif5c; KIF5C protein; KIF5C_HUMAN; Kinesin family member 5C; Kinesin heavy chain isoform 5C; Kinesin heavy chain neuron specific 2; Kinesin heavy chain neuron specific; Kinesin heavy chain neuron-specific 2; Kinesin5C; KINN; MGC111478; NKHC 2; NKHC; NKHC-2; NKHC2; anti-KIF5C antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat; Predicted Reactivity: Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Kinesin 5 C Antibody detects endogenous levels of total Kinesin 5 C.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 957

• Applicable Applications for anti-KIF5C antibody: ELISA (EIA)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200; ELISA(peptide): 1:20,000-1:40,000
• Immunogen: A synthesized peptide derived from human Kinesin 5 C, corresponding to a region within C-terminal amino acids.
• Fragment: Fab fragment
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of extracts from Rat brain, using Kinesin 5 C Antibody. The lane on the left was treated with blocking peptide.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612315 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Immunohistochemistry (IHC)-Paraffin

(MBS9612315 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

Related Product Information for anti-KIF5C antibody

Function: Involved in synaptic transmission (PubMed:24812067). Mediates dendritic trafficking of mRNAs (By similarity). Kinesin is a microtubule-associated force-producing protein that may play a role in organelle transport. Required for anterograde axonal transportation of MAPK8IP3/JIP3 which is essential for MAPK8IP3/JIP3 function in axon elongation (By similarity).

Subcellular Location: Cytoplasm>Cytoskeleton. Cell projection>Dendrite. Note: Abundant in distal regions of dendrites.

Tissue Specificity: Highest expression in brain, prostate and testis, and moderate expression in kidney, small intestine and ovary.

Subunit Structure: Oligomer composed of two heavy chains and two light chains. Interacts with GRIP1 and KLC3 (By similarity). Interacts with TRAK1 (PubMed:15644324). Interacts with ZFYVE27 (By similarity).

Similarity: Composed of three structural domains: a large globular N-terminal domain which is responsible for the motor activity of kinesin (it hydrolyzes ATP and binds microtubule), a central alpha-helical coiled coil domain that mediates the heavy chain dimerization; and a small globular C-terminal domain which interacts with other proteins (such as the kinesin light chains), vesicles and membranous organelles.Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family. Kinesin subfamily.

NCBI and Uniprot Product Information

• NCBI GI #: 4758650
• NCBI GeneID: 3800
• NCBI Accession #: NP_004513.1
• NCBI GenBank Nucleotide #: NP_004513.1
• UniProt Accession #: O60282
• Molecular Weight: Observed Molecular Weight: (Observed)109kD.; Predicted Molecular Weight: (Calculated)109kDa.
• NCBI Official Full Name: kinesin heavy chain isoform 5C
• NCBI Official Synonym Full Names: kinesin family member 5C
• NCBI Official Symbol: KIF5C
• NCBI Official Synonym Symbols: KINN; NKHC; NKHC2; CDCBM2; NKHC-2
• NCBI Protein Information: kinesin heavy chain isoform 5C; kinesin heavy chain
• UniProt Protein Name: Kinesin heavy chain isoform 5C
• Protein Family: Kinesin
• UniProt Gene Name: KIF5C
• UniProt Synonym Gene Names: KIAA0531; NKHC2
• UniProt Entry Name: KIF5C_HUMAN

NCBI Description

The protein encoded by this gene is a kinesin heavy chain subunit involved in the transport of cargo within the central nervous system. The encoded protein, which acts as a tetramer by associating with another heavy chain and two light chains, interacts with protein kinase CK2. Mutations in this gene have been associated with complex cortical dysplasia with other brain malformations-2. Two transcript variants, one protein-coding and the other non-protein coding, have been found for this gene. [provided by RefSeq, Jul 2015]