Rabbit anti-Human KIN Polyclonal Antibody | anti-KIN antibody

KIN Antibody

Cat. Num. AAA9629261

• Gene Names: KIN; BTCD; Rts2; KIN17
• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KIN; Polyclonal Antibody; KIN Antibody; antigenic determinant of recA protein homolog; Binding to curved DNA; BTCD; DNA/RNA-binding protein KIN17; HsKin17 protein; KIN antigenic determinant of recA protein homolog mouse; Kin17 protein; KIN17_HUMAN; anti-KIN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KIN Antibody detects endogenous levels of total KIN.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KIN antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from Human MYOCD.
• Conjugation: Unconjugated
• Similarity: The C-terminal domain (268-393) is organized into 2 subdomains that bear structural similarities to SH3-like domains. Both subdomains adopt a similar 5-stranded beta-barrel-like fold and are connected to each other by a short linker of 5 residues. The 5 beta-sheets are packed at approximately right angles against each other. A highly conserved groove formed at the interface between the 2 subdomains, comprised of Lys residues 302 and 391 and other positively charged residues, may possibly be the site of RNA-binding. Belongs to the KIN17 family.
• Subunit Structure: Associated with DNA polymerase alpha, RFC1 and cyclin A, in multiprotein DNA replication complexes. Also associates with replication origins at the G1/S phase boundary and throughout the S phase in vivo.(Microbial infection) Interacts with SV40 large T antigen.
• Tissue Specificity: Ubiquitously expressed in all tissues examined, with highest levels in skeletal muscle, heart and testis. Differentially expressed in non-tumorigenic and tumorigenic cell lines. Highly expressed in proliferating epithelial keratinocyte cells in vitro (at protein level).
• Function: Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo (By similarity). Binds via its C-terminal domain to RNA in vitro.
• Note: During S phase, strongly associated with the nuclear matrix, and to chromosomal DNA in the presence of DNA damage. Also shows cytoplasmic localization in elongated spermatids.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-KIN antibody

Nucleus; Cytoplasm; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 74762109
• NCBI GeneID: 22944
• UniProt Accession #: O60870; B4DX32
• Molecular Weight: 393
• NCBI Official Full Name: DNA/RNA-binding protein KIN17
• NCBI Official Synonym Full Names: Kin17 DNA and RNA binding protein
• NCBI Official Symbol: KIN
• NCBI Official Synonym Symbols: BTCD; Rts2; KIN17
• NCBI Protein Information: DNA/RNA-binding protein KIN17; binding to curved DNA; KIN, antigenic determinant of recA protein homolog
• UniProt Protein Name: DNA/RNA-binding protein KIN17
• Protein Family: Kinin
• UniProt Gene Name: KIN
• UniProt Entry Name: KIN17_HUMAN

NCBI Description

The protein encoded by this gene is a nuclear protein that forms intranuclear foci during proliferation and is redistributed in the nucleoplasm during the cell cycle. Short-wave ultraviolet light provokes the relocalization of the protein, suggesting its participation in the cellular response to DNA damage. Originally selected based on protein-binding with RecA antibodies, the mouse protein presents a limited similarity with a functional domain of the bacterial RecA protein, a characteristic shared by this human ortholog. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2012]

Uniprot Description

KIN: Involved in DNA replication and the cellular response to DNA damage. May participate in DNA replication factories and create a bridge between DNA replication and repair mediated by high molecular weight complexes. May play a role in illegitimate recombination and regulation of gene expression. May participate in mRNA processing. Binds, in vitro, to double-stranded DNA. Also shown to bind preferentially to curved DNA in vitro and in vivo. Binds via its C-terminal domain to RNA in vitro. Belongs to the KIN17 family.

Protein type: C2H2-type zinc finger protein; DNA replication

Chromosomal Location of Human Ortholog: 10p14

Cellular Component: nuclear matrix; intracellular membrane-bound organelle; cytoplasm; nucleus

Molecular Function: DNA binding; RNA binding; metal ion binding; double-stranded DNA binding

Biological Process: viral reproduction; DNA replication; mRNA processing; DNA repair; response to DNA damage stimulus; DNA recombination