Rabbit KIF5 Polyclonal Antibody | anti-KIF5A antibody

KIF5 Antibody

Cat. Num. AAA150427

• Gene Names: KIF5A; NKHC; MY050; SPG10; D12S1889
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence
• Purity: KIF5 has at least three known isoforms; this antibody recognizes all three.
• Synonyms: KIF5; Polyclonal Antibody; KIF5 Antibody; NKHC; MY050; SPG10; D12S1889; NKHC1; Kinesin heavy chain isoform 5A; Kinesin heavy chain neuron-specific 1; kinesin family member 5A; anti-KIF5A antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: KIF5 has at least three known isoforms; this antibody recognizes all three.
• Form/Format: Liquid; KIF5 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/mL (varies by lot)
• Sequence Length: 1032

• Applicable Applications for anti-KIF5A antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF)
• Application Notes: KIF5 antibody can be used for detection of KIF5 by Western blot at 0.5 - 1 ug/mL. Antibody can also be used for immunocytochemistry starting at 5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Conjugate: Unconjugated
• Immunogen: KIF5 antibody was raised against a 20 amino acid synthetic peptide from near the center of human KIF5.; The immunogen is located within amino acids 330 - 380 of KIF5.
• Positive Control: 1) Cat. No. MBS151739 - K562 Cell Lysate; 2) Cat. No. MBS154303 - K-562 Cell Slide
• Preparation and Storage: KIF5 antibody can be stored at 4 degree C for three months and -20 degree C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of KIF5 in K562 cell lysate with KIF5 antibody at (A) 0.5 and (B) 1 μg/mL.)

Immunocytochemistry (IHC)

(Immunocytochemistry of KIF5 in K562 cells with KIF5 antibody at 5 μg/mL.)

Immunofluorescence (IF)

(Immunofluorescence of KIF5 in K562 cells with KIF5 antibody at 20 μg/mL.)

Related Product Information for anti-KIF5A antibody

KIF5 Antibody: The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

NCBI and Uniprot Product Information

• NCBI GI #: 143811412
• NCBI GeneID: 3798
• UniProt Accession #: Q12840; Q4LE26; A6H8M5
• Molecular Weight: 117,378 Da
• NCBI Official Full Name: Kinesin heavy chain isoform 5A
• NCBI Official Synonym Full Names: kinesin family member 5A
• NCBI Official Symbol: KIF5A
• NCBI Official Synonym Symbols: NKHC; MY050; SPG10; D12S1889
• NCBI Protein Information: kinesin heavy chain isoform 5A; KIF5A variant protein; neuronal kinesin heavy chain; kinesin heavy chain neuron-specific 1; kinesin, heavy chain, neuron-specific
• UniProt Protein Name: Kinesin heavy chain isoform 5A
• Protein Family: Kinesin
• UniProt Gene Name: KIF5A
• UniProt Synonym Gene Names: NKHC1; NKHC
• UniProt Entry Name: KIF5A_HUMAN

NCBI Description

This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF5A: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10). An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Belongs to the kinesin-like protein family. Kinesin subfamily.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: kinesin complex; microtubule; membrane; perinuclear region of cytoplasm; cytosol

Molecular Function: protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; motor activity; ATP binding

Biological Process: synaptic transmission; axon guidance; protein localization; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytoskeleton-dependent intracellular transport; blood coagulation; microtubule-based movement

Disease: Spastic Paraplegia 10, Autosomal Dominant