Rabbit KIF5 Polyclonal Antibody | anti-KIF5 antibody

KIF5 Antibody

Cat. Num. AAA9405988

• Gene Names: KIF5A; NKHC; MY050; SPG10; D12S1889
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunocytochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: KIF5; Polyclonal Antibody; KIF5 Antibody; Kinesin heavy chain isoform 5; neuronal kinesin heavy chain; NKHC; anti-KIF5 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 1032

• Applicable Applications for anti-KIF5 antibody: ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 20 amino acid peptide from near the carboxy terminus of human KIF5.
• Target Name: KIF5
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of KIF5 in K562 cell lysate with KIF5 antibody at (A) 0.5 and (B) 1 ug/mL.)

Immunocytochemistry (ICC)

(Immunocytochemistry of KIF5 in K562 cells with KIF5 antibody at 5 ug/mL.)

Related Product Information for anti-KIF5 antibody

The kinesin superfamily proteins (KIFs) are microtubule-dependent motors that slide along microtubules and transport cellular organelles and mRNA to different parts of the cell. In neurons, KIF5 performs this role in an anterograde fashion from the neuronal cell body to axonal or dendritic terminals. KIF5 was initially differentiated from the ubiquitously expressed kinesin as being highly enriched in subsets of neurons and selectively concentrated in the cell body. KIF5 has at least three known isoforms; this antibody recognizes all three. Mutations in one of the isoforms of KIF5 (KIF5A) are thought to be involved in hereditary spastic paraplegias.

Product Categories/Family for anti-KIF5 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 45446749
• NCBI GeneID: 3798
• NCBI Accession #: NP_004975.2
• NCBI GenBank Nucleotide #: NM_004984.2
• UniProt Accession #: Q12840; Q4LE26; A6H8M5
• Molecular Weight: 117,378 Da
• NCBI Official Full Name: kinesin heavy chain isoform 5A
• NCBI Official Synonym Full Names: kinesin family member 5A
• NCBI Official Symbol: KIF5A
• NCBI Official Synonym Symbols: NKHC; MY050; SPG10; D12S1889
• NCBI Protein Information: kinesin heavy chain isoform 5A
• UniProt Protein Name: Kinesin heavy chain isoform 5A
• Protein Family: Kinesin-related protein
• UniProt Gene Name: KIF5A
• UniProt Synonym Gene Names: NKHC1; NKHC
• UniProt Entry Name: KIF5A_HUMAN

NCBI Description

This gene encodes a member of the kinesin family of proteins. Members of this family are part of a multisubunit complex that functions as a microtubule motor in intracellular organelle transport. Mutations in this gene cause autosomal dominant spastic paraplegia 10. [provided by RefSeq, Jul 2008]

Uniprot Description

KIF5A: Microtubule-dependent motor required for slow axonal transport of neurofilament proteins (NFH, NFM and NFL). Defects in KIF5A are the cause of spastic paraplegia autosomal dominant type 10 (SPG10). An inherited degenerative spinal cord disorder characterized by a slow, gradual, progressive weakness and spasticity (stiffness) of the legs. Rate of progression and the severity of symptoms is quite variable. Initial symptoms may include difficulty with balance, weakness and stiffness in the legs, muscle spasms, and dragging the toes when walking. In some forms of the disorder, bladder symptoms (such as incontinence) may appear, or the weakness and stiffness may spread to other parts of the body. Belongs to the kinesin-like protein family. Kinesin subfamily.

Protein type: Motor; Microtubule-binding

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: kinesin complex; microtubule; membrane; perinuclear region of cytoplasm; cytosol

Molecular Function: protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; motor activity; ATP binding

Biological Process: axon guidance; synaptic transmission; protein localization; metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class II; cytoskeleton-dependent intracellular transport; blood coagulation; microtubule-based movement

Disease: Spastic Paraplegia 10, Autosomal Dominant