Rabbit anti-Human KIF14 Polyclonal Antibody | anti-KIF14 antibody

KIF14 Antibody

Cat. Num. AAA9631011

• Reactivity: Human
• Applications: ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Synonyms: KIF14; Polyclonal Antibody; KIF14 Antibody; KIAA0042; KIF 14; KIF14_HUMAN; Kinesin Family Member 14; Kinesin like protein KIF14; Kinesin-like protein KIF14; MGC142302; anti-KIF14 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: KIF14 Antibody detects endogenous levels of KIF14.
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific).
• Form/Format: Liquid. Rabbit IgG in phosphate buffered saline, pH7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-KIF14 antibody: ELISA (EIA)(peptide)
• Application Notes: ELISA(peptide): 1:20000-1:40000
• Immunogen: A synthesized peptide derived from human KIF14.
• Conjugation: Unconjugated
• Similarity: The kinesin motor domain binds to microtubules with high affinity and has a robust ATPase activity but a very slow motility. The kinesin motor domain protects microtubules from cold depolymerization. Binds to each tubulin heterodimer resulting in a microtubule complexes. Binds at the tubulin intradimer interface, at the crest of the protofilament, and orients slightly toward the next protofilament. Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.
• Subunit Structure: Directly interacts with PRC1 within a complex also containing KIF4A, KIF20A and KIF23; targets to the central spindle. Directly interacts with CIT depending on the activation state of the kinase (stronger interaction with the kinase-dead form); targets to the midbody. Interacts with ARRB2; the interaction is detected in the nucleus upon OR1D2 stimulation. Interacts with AKT1; the interaction is detected in the plasma membrane upon INS stimulation and promotes AKT1 phosphorylation. Interacts with SVIL; at midbody during cytokinesis. Interacts with RADIL (via PDZ domain); recruits RADIL to the microtubule network restricting RADIL from interaction with activated RAP1A.
• Function: Microtubule motor protein that binds to microtubules with high affinity through each tubulin heterodimer and has an ATPase activity (By similarity). Plays a role in many processes like cell division, cytokinesis and also in cell proliferation and apoptosis. During cytokinesis, targets to central spindle and midbody through its interaction with PRC1 and CIT respectively. Regulates cell growth through regulation of cell cycle progression and cytokinesis. During cell cycle progression acts through SCF-dependent proteasomal ubiquitin-dependent protein catabolic process which controls CDKN1B degradation, resulting in positive regulation of cyclins, including CCNE1, CCND1 and CCNB1. During late neurogenesis, regulates the cerebellar, cerebral cortex and olfactory bulb development through regulation of apoptosis, cell proliferation and cell division (By similarity). Also is required for chromosome congression and alignment during mitotic cell cycle process. Regulates cell spreading, focal adhesion dynamics, and cell migration through its interaction with RADIL resulting in regulation of RAP1A-mediated inside-out integrin activation by tethering RADIL on microtubules.
• Note: Nuclear localization observed during interphase (PubMed:16431929). Nuclear localization triggered by entry into mitosis (PubMed:16648480). Cytoplasmic in interphase (PubMed:16648480). Cytoplasmic in metaphase cells (PubMed:16431929). From prophase to metaphase, accumulates at the developing spindle poles and their associated microtubules. During anaphase, accumulates at the spindle midzone. Localization to the central spindle and midbody during anaphase is dependent upon PRC1 and CIT presence. In cells ready to undergo abscission, concentrates at the contractile ring.
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Product Categories/Family for anti-KIF14 antibody

Nucleus; Cytoplasm; Cytoskeleton; Spindle; Midbody; Primary

NCBI and Uniprot Product Information

• NCBI GI #: 119611721
• NCBI GeneID: 9928
• UniProt Accession #: Q15058; Q14CI8; Q4G0A5; Q5T1W3
• Molecular Weight: 186,492 Da
• NCBI Official Full Name: kinesin family member 14, isoform CRA_a
• NCBI Official Synonym Full Names: kinesin family member 14
• NCBI Official Symbol: KIF14
• NCBI Protein Information: kinesin-like protein KIF14
• UniProt Protein Name: Kinesin-like protein KIF14
• Protein Family: Kinesin-like protein
• UniProt Gene Name: KIF14
• UniProt Synonym Gene Names: KIAA0042
• UniProt Entry Name: KIF14_HUMAN

NCBI Description

KIF14 is a member of the kinesin superfamily of microtubule-associated motors (see MIM 148760) that play important roles in intracellular transport and cell division (Nakagawa et al., 1997 [PubMed 9275178]).[supplied by OMIM, Mar 2008]

Uniprot Description

KIF14: Plays an essential role in cytokinesis. Belongs to the kinesin-like protein family.

Protein type: Microtubule-binding; Motor

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: microtubule; kinesin complex; membrane; cytoplasm; plasma membrane; spindle midzone; midbody; nucleus

Molecular Function: tubulin binding; protein binding; plus-end-directed microtubule motor activity; microtubule binding; ATPase activity; microtubule motor activity; protein kinase binding; ATP binding; PDZ domain binding

Biological Process: positive regulation of cytokinesis; regulation of Rap protein signal transduction; regulation of cell adhesion; SCF-dependent proteasomal ubiquitin-dependent protein catabolic process; proteasomal ubiquitin-dependent protein catabolic process; cerebellar granular layer structural organization; regulation of myelination; cell proliferation in forebrain; olfactory bulb development; hippocampus development; cytoskeleton-dependent intracellular transport; negative regulation of integrin activation; regulation of cell migration; mitotic metaphase plate congression; microtubule-based movement; cerebellar cortex development; establishment of protein localization; regulation of G2/M transition of mitotic cell cycle; regulation of neuron apoptosis; positive regulation of cell proliferation; activation of protein kinase activity; microtubule depolymerization; negative regulation of neuron apoptosis; cerebral cortex development; regulation of cell growth; cerebellar Purkinje cell layer structural organization; negative regulation of apoptosis

Disease: Meckel Syndrome 12