Western Blot (WB)
(Anti- KIAA1524 Picoband antibody, MBS178137, Western blottingAll lanes: Anti KIAA1524 (MBS178137) at 0.5ug/mlLane 1: Mouse Testis Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugPredicted bind size: 102KDObserved bind size: 90KD )
anti-Human, Mouse KIAA1524 Polyclonal Antibody | anti-KIAA1524 antibody
Anti-KIAA1524 Antibody
Gene Names
KIAA1524; p90; CIP2A
Reactivity
Human, Mouse
Applications
Western Blot
Purity
Immunogen Affinity Purified
Synonyms
KIAA1524; Polyclonal Antibody; Anti-KIAA1524 Antibody; Protein CIP2A; Cancerous inhibitor of PP2A; Cancerous Inhibitor of Protein Phosphatase 2A; CIP2A; CIP2A_HUMAN; FLJ12850; Kiaa1524; MGC163436; p90; p90 autoantigen; anti-KIAA1524 antibody
Reactivity
Human, Mouse
Clonality
Polyclonal
Purity/Purification
Immunogen Affinity Purified
Form/Format
Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.
Sequence Length
905
Applicable Applications for anti-KIAA1524 antibody
Western Blot (WB)
Application Notes
Western Blot Concentration: 0.1-0.5ug/ml
Immunogen
A synthetic peptide corresponding to a sequence at the C-terminus of human KIAA1524 (854-889aa EVQKAQLEGRLEEKESLVKLQQEELNKHSHMIAMIH), different from the related mouse sequence by three amino acids.
Ig Type
Rabbit IgG
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
Preparation and Storage
At -20 degree C for one year. After reconstitution, at 4 degree C for one month. It can also be aliquoted and stored frozen at -20 degree C for a longer time. Avoid repeated freezing and thawing.
Western Blot (WB)
(Anti- KIAA1524 Picoband antibody, MBS178137, Western blottingAll lanes: Anti KIAA1524 (MBS178137) at 0.5ug/mlLane 1: Mouse Testis Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugPredicted bind size: 102KDObserved bind size: 90KD )
Western Blot (WB)
(Anti- KIAA1524 Picoband antibody, MBS178137, Western blottingAll lanes: Anti KIAA1524 (MBS178137) at 0.5ug/mlLane 1: Mouse Testis Tissue Lysate at 50ugLane 2: HELA Whole Cell Lysate at 40ugPredicted bind size: 102KDObserved bind size: 90KD )
Related Product Information for anti-KIAA1524 antibody
Description: Rabbit IgG polyclonal antibody for Protein CIP2A(KIAA1524) detection. Tested with WB in Human;Mouse.
Background: Protein CIP2A, also known as cancerous inhibitor of PP2A (CIP2A), is a protein that in humans is encoded by the KIAA1524 gene. CIP2A is required for the malignant cellular growth and for in vivo tumor formation. In accordance with the oncogenic role of CIP2A, overexpression of CIP2A promotes Ras-elicited cell growth and transforms immortalized human cells (HEK-TERVs). More recently CIP2A has been shown to regulate phosphorylation and activity of many other oncoproteins and to drive malignant cell growth and tumorigenesis in various human cancer types. Importantly, CIP2A deficient mice are viable, suggesting that targeting of oncogenic function of CIP2A would not result in serious side-effects.
Background: Protein CIP2A, also known as cancerous inhibitor of PP2A (CIP2A), is a protein that in humans is encoded by the KIAA1524 gene. CIP2A is required for the malignant cellular growth and for in vivo tumor formation. In accordance with the oncogenic role of CIP2A, overexpression of CIP2A promotes Ras-elicited cell growth and transforms immortalized human cells (HEK-TERVs). More recently CIP2A has been shown to regulate phosphorylation and activity of many other oncoproteins and to drive malignant cell growth and tumorigenesis in various human cancer types. Importantly, CIP2A deficient mice are viable, suggesting that targeting of oncogenic function of CIP2A would not result in serious side-effects.
References
1. Dong QZ, Wang Y, Dong XJ, Li ZX, Tang ZP, Cui QZ, Wang EH (September 2010). "CIP2A is Overexpressed in Non-Small Cell Lung Cancer and Correlates with Poor Prognosis". Ann Surg Oncol 18 (3): 857-865. 2. Junttila MR, Puustinen P, Niemelä M, et al. (2007). "CIP2A inhibits PP2A in human malignancies". Cell 130 (1): 51-62. 3. Khanna A, Böckelman C, Hemmes A, Junttila MR, Wiksten JP, Lundin M, Junnila S, Murphy DJ, Evan GI, Haglund C, Westermarck J, Ristimäki A (June 2009). "MYC-dependent regulation and prognostic role of CIP2A in gastric cancer". J. Natl. Cancer Inst. 101 (11): 793-805.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
84,579 Da
NCBI Official Full Name
protein CIP2A
NCBI Official Synonym Full Names
KIAA1524
NCBI Official Symbol
KIAA1524
NCBI Official Synonym Symbols
p90; CIP2A
NCBI Protein Information
protein CIP2A
UniProt Protein Name
Protein CIP2A
Protein Family
UniProt Gene Name
KIAA1524
UniProt Synonym Gene Names
CIP2A
UniProt Entry Name
CIP2A_HUMAN
Uniprot Description
CIP2A: an oncoprotein that inhibits PP2A in human malignancies. Like the SV40 small t antigen, inhibits the phospho-serine/-threonine phosphatase PP2A. Promotes c-Myc protein stability by inhibiting the dephosphorylation of phospho-serine 62. Expressed at low levels in most of nonmalignant tissues but at high levels in human malignancies including head and neck squamous cell carcinoma (HNSCC), colon cancer, and liver cancer. Antibodies against CIP2A are present in sera from many patients with gastric or prostate cancer. Two alternatively spliced human isoforms have been described.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 3q13.13
Cellular Component: cytoplasm; integral to membrane; plasma membrane
Molecular Function: protein binding
Biological Process: spermatogenesis
Research Articles on KIAA1524
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Product Notes
The KIAA1524 kiaa1524 (Catalog #AAA178137) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-KIAA1524 Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's KIAA1524 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Western Blot Concentration: 0.1-0.5ug/ml. Researchers should empirically determine the suitability of the KIAA1524 kiaa1524 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIAA1524, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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