Rabbit KHDRBS3 Polyclonal Antibody | anti-KHDRBS3 antibody

KHDRBS3 Antibody

Cat. Num. AAA9609915

• Gene Names: KHDRBS3; Etle; SALP; SLM2; SLM-2; TSTAR; T-STAR; etoile
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, ELISA
• Purity: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Synonyms: KHDRBS3; Polyclonal Antibody; KHDRBS3 Antibody; Etle; etoile; KH domain containing RNA binding signal transduction associated 3; KH domain containing RNA binding signal transduction associated protein 3; KH domain-containing; KHDR3_HUMAN; Khdrbs3; RNA binding protein T Star; RNA-binding; RNA-binding protein T-Star; SALP; Sam68 like mammalian protein 2; Sam68 like phosphotyrosine protein; Sam68 like phosphotyrosine protein T STAR; Sam68-like mammalian protein 2; Sam68-like phosphotyrosine protein; signal transduction-associated protein 3; SLM 2; SLM-2; SLM2; T STAR; TSTAR; anti-KHDRBS3 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: KHDRBS3 antibody detects endogenous levels of total KHDRBS3
• Purity/Purification: The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
• Form/Format: Liquid; Phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1mg/ml (varies by lot)
• Sequence Length: 346

• Applicable Applications for anti-KHDRBS3 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-3000
• Immunogen: A synthesized peptide derived from human KHDRBS3
• Subcellular Location: Nucleus. Localized in a compartment adjacent to the nucleolus, but distinct from the peri-nucleolar one.
• Tissue Specificity: Ubiquitous with higher expression in testis, skeletal muscle and brain. Expressed in the kidney only in podocytes, the glomerular epithelial cells of the kidney. Strongly expressed after meiosis.
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C. Stable for 12 months from date of receipt.

Western Blot (WB)

(Western blot analysis of KHDRBS3 using Jurkat whole cell lysates)

Related Product Information for anti-KHDRBS3 antibody

Function: RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Binds optimally to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). RNA-binding abilities are down-regulated by tyrosine kinase PTK6 (PubMed:10564820, PubMed:19561594, PubMed:26758068). Involved in splice site selection of vascular endothelial growth factor (PubMed:15901763). In vitro regulates CD44 alternative splicing by direct binding to purine-rich exonic enhancer (By similarity). Can regulate alternative splicing of neurexins NRXN1-3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners such as neuroligins and LRRTM family members (PubMed:26758068). Targeted, cell-type specific splicing regulation of NRXN1 at AS4 is involved in neuronal glutamatergic synapse function and plasticity (By similarity). May regulate expression of KHDRBS2/SLIM-1 in defined brain neuron populations by modifying its alternative splicing (By similarity). Can bind FABP9 mRNA (By similarity). May play a role as a negative regulator of cell growth. Inhibits cell proliferation.
Subunit Structure: Self-associates to form homooligomers; dimerization increases RNA affinity (PubMed:26758068). Interacts with KHDRBS2/SLM-1 (By similarity). Interacts with KHDRBS1/SAM68; heterooligomer formation of KHDRBS family proteins may modulate RNA substrate specificity (PubMed:10332027). Interacts with the splicing regulatory proteins SFRS9, SAFB and YTHDC1. Interacts with HNRPL (By similarity). Interacts with RBMX, RBMY1A1, p85 subunit of PI3-kinase, SERPINB5 (PubMed:10564820, PubMed:10332027, PubMed:10749975, PubMed:21725612). Interacts with SIAH1 which promotes targeting for degradation (PubMed:15163637).
Post-translational Modifications: Phosphorylated on tyrosine residues. Isoform 1 C-terminal region is tyrosine-rich, but isoform 2 lacking this C-terminal region is also tyrosine-phosphorylated.
Similarity: The proline-rich site binds the SH3 domain of the p85 subunit of PI3-kinase. Belongs to the KHDRBS family.

NCBI and Uniprot Product Information

• NCBI GI #: 5730073
• NCBI GeneID: 10656
• NCBI Accession #: NP_006549.1
• NCBI GenBank Nucleotide #: NM_006558.2
• UniProt Accession #: O75525; Q6NUL8; Q9UPA8
• Molecular Weight: Observed: 39 kDa; Predicted: 39 kDa
• NCBI Official Full Name: KH domain-containing, RNA-binding, signal transduction-associated protein 3
• NCBI Official Synonym Full Names: KH RNA binding domain containing, signal transduction associated 3
• NCBI Official Symbol: KHDRBS3
• NCBI Official Synonym Symbols: Etle; SALP; SLM2; SLM-2; TSTAR; T-STAR; etoile
• NCBI Protein Information: KH domain-containing, RNA-binding, signal transduction-associated protein 3
• UniProt Protein Name: KH domain-containing, RNA-binding, signal transduction-associated protein 3
• Protein Family: KH domain-containing, RNA-binding, signal transduction-associated protein
• UniProt Gene Name: KHDRBS3
• UniProt Synonym Gene Names: SALP; SLM2; SLM-2

Uniprot Description

RNA-binding protein that plays a role in the regulation of alternative splicing and influences mRNA splice site selection and exon inclusion. Binds preferentially to the 5'-[AU]UAAA-3' motif in vitro. Binds optimally to RNA containing 5'-[AU]UAA-3' as a bipartite motif spaced by more than 15 nucleotides. Binds poly(A). RNA-binding abilities are down-regulated by tyrosine kinase PTK6 (PubMed:10564820, PubMed:19561594, PubMed:26758068). Involved in splice site selection of vascular endothelial growth factor (PubMed:15901763). In vitro regulates CD44 alternative splicing by direct binding to purine-rich exonic enhancer (). Can regulate alternative splicing of neurexins NRXN1-3 in the laminin G-like domain 6 containing the evolutionary conserved neurexin alternative spliced segment 4 (AS4) involved in neurexin selective targeting to postsynaptic partners such as neuroligins and LRRTM family members (PubMed:26758068). Targeted, cell-type specific splicing regulation of NRXN1 at AS4 is involved in neuronal glutamatergic synapse function and plasticity (). May regulate expression of KHDRBS2/SLIM-1 in defined brain neuron populations by modifying its alternative splicing (). Can bind FABP9 mRNA (). May play a role as a negative regulator of cell growth. Inhibits cell proliferation.