Rabbit KGF Polyclonal Antibody | anti-KGF antibody

RABBIT ANTI HUMAN KGF

Cat. Num. AAA221721

• Gene Names: FGF7; KGF; HBGF-7
• Applications: ELISA, Functional Assay, Immunohistochemistry, Western Blot
• Synonyms: KGF; Polyclonal Antibody; RABBIT ANTI HUMAN KGF; anti-KGF antibody

• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - lyophilised
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 194

• Applicable Applications for anti-KGF antibody: ELISA (EIA), Functional Assays (FN), Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: ELISA: Maximum Dilution: 0.5ug/ml;; Functional Assays: Minimum Dilution: 0.56; Maximum Dilution: 0.85ug/ml;; Immunohistology - Paraffin: Application Note: This product requires enzyme mediated antigen retrieval prior to staining of parrafin sections. Proteinase K is recomended for this purpose. ; ; Western Blotting: Minimum Dilution: 0.1; Maximum Dilution: 0.2ug/ml
• Reconstitution: Reconstitute with 0.1ml distilled water. For long term storage the addition of 0.09% sodium azide is recommended. NB: For functional studies do not add azide.
• Immunogen: Recombinant human KGF; Buffer Solution
• Antiserum Preparation: Antisera to human KGF were raised by repeated immunisations of rabbits with highly purified antigen. Purified IgG prepared by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Prior to reconstitution store at 4 degree C. After reconstitution store at -20 degree C. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human KGF (MBS221721) following antigen retrieval using proteinase K)

Testing Data #2

(Formalin fixed, paraffin embedded normal human skin stained with Rabbit anti Human KGF (MBS221721) following antigen retrieval using proteinase K)

Related Product Information for anti-KGF antibody

Rabbit anti Human KGF antibody recognizes human KGF (keratinocyte growth factor), a member of the fibroblast growth factor family, which acts as a potent mitogen of epithelial cells, predominantly of keratinocyte origin. KGF is secreted by fibroblasts in response to stimulation from inflammatory mediators, including IL-1 beta and PDGF-BB (platelet-derived growth factor B), and binds specifically to a splice variant of the FGF receptor 2 (FGFR2-IIIb), known as the KGF receptor.

NCBI and Uniprot Product Information

• NCBI GI #: 4503705
• NCBI GeneID: 2252
• NCBI Accession #: NP_002000.1
• NCBI GenBank Nucleotide #: NM_002009.3
• UniProt Accession #: P21781; Q6FGV5; Q96FG5; H0YNY5
• Molecular Weight: 11,520 Da
• NCBI Official Full Name: fibroblast growth factor 7
• NCBI Official Synonym Full Names: fibroblast growth factor 7
• NCBI Official Symbol: FGF7
• NCBI Official Synonym Symbols: KGF; HBGF-7
• NCBI Protein Information: fibroblast growth factor 7; FGF-7; heparin-binding growth factor 7; keratinocyte growth factor
• UniProt Protein Name: Fibroblast growth factor 7
• UniProt Gene Name: FGF7
• UniProt Synonym Gene Names: KGF; FGF-7; HBGF-7
• UniProt Entry Name: FGF7_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein is a potent epithelial cell-specific growth factor, whose mitogenic activity is predominantly exhibited in keratinocytes but not in fibroblasts and endothelial cells. Studies of mouse and rat homologs of this gene implicated roles in morphogenesis of epithelium, reepithelialization of wounds, hair development and early lung organogenesis. [provided by RefSeq, Jul 2008]

Uniprot Description

FGF7: Plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. Required for normal branching morphogenesis. Growth factor active on keratinocytes. Possible major paracrine effector of normal epithelial cell proliferation. Belongs to the heparin-binding growth factors family.

Protein type: Secreted; Cytokine; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 15q21.2

Cellular Component: Golgi apparatus; extracellular region

Molecular Function: heparin binding; protein binding; growth factor activity; chemoattractant activity; fibroblast growth factor receptor binding

Biological Process: positive regulation of keratinocyte migration; epidermal growth factor receptor signaling pathway; epidermis development; hair follicle morphogenesis; phosphoinositide-mediated signaling; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; mesenchymal cell proliferation; signal transduction; positive regulation of peptidyl-tyrosine phosphorylation; positive chemotaxis; positive regulation of cell division; positive regulation of cell proliferation; actin cytoskeleton reorganization; response to wounding; insulin receptor signaling pathway; innate immune response; positive regulation of epithelial cell proliferation