Western Blot (WB)
(Western blotAll lanes: Keratin, type I cytoskeletal 18 antibody at 2ug/mlLane 1: K562 whole cell lysateLane 2: HepG2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)
Rabbit anti-Human Keratin, type I cytoskeletal 18 Polyclonal Antibody | anti-KRT18 antibody
Rabbit anti-Human Keratin, type I cytoskeletal 18 polyclonal Antibody
Western Blot (WB)
(Western blotAll lanes: Keratin, type I cytoskeletal 18 antibody at 2ug/mlLane 1: K562 whole cell lysateLane 2: HepG2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)
Western Blot (WB)
(Western blotAll lanes: Keratin, type I cytoskeletal 18 antibody at 2ug/mlLane 1: K562 whole cell lysateLane 2: HepG2 whole cell lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48kDaObserved band size: 48kDa)
Western Blot (WB)
(Western blot All lanes: KRT18 antibody at 12ug/mlLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48 kDaObserved band size: 48,66 kDa)
Western Blot (WB)
(Western blot All lanes: KRT18 antibody at 12ug/mlLane 1: A431 whole cell lysateLane 2: Hela whole cell lysateLane 3: Jurkat whole cell lysateLane 4: Zebrafish lysateSecondaryGoat polyclonal to Rabbit IgG at 1/10000 dilutionPredicted band size: 48 kDaObserved band size: 48,66 kDa)
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate,Hela whole cell lysateAll lanes: KRT18 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49 KDaObserved band size: 49 KDa)
Western Blot (WB)
(Western BlotPositive WB detected in: K562 whole cell lysate,Hela whole cell lysateAll lanes: KRT18 antibody at 3ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 49 KDaObserved band size: 49 KDa)
Immunohistochemistry (IHC)
(IHC image of MBS7000063 diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunohistochemistry (IHC)
(IHC image of MBS7000063 diluted at 1:800 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS7000063 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
Immunofluorescence (IF)
(Immunofluorescent analysis of HepG2 cells using MBS7000063 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L))
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
NCBI and Uniprot Product Information
NCBI Description
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
Uniprot Description
K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.
Protein type: Cytoskeletal; Nucleolus
Chromosomal Location of Human Ortholog: 12q13
Cellular Component: cytoplasm; intermediate filament; keratin filament; microtubule organizing center; nucleolus; perinuclear region of cytoplasm
Molecular Function: protein binding; structural molecule activity
Biological Process: anatomical structure morphogenesis; cell cycle; Golgi to plasma membrane CFTR protein transport; intermediate filament cytoskeleton organization and biogenesis; negative regulation of apoptosis; tumor necrosis factor-mediated signaling pathway; viral reproduction
Disease: Cirrhosis, Familial
Research Articles on KRT18
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Product Notes
The KRT18 krt18 (Catalog #AAA7000063) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-Human Keratin, type I cytoskeletal 18 polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin, type I cytoskeletal 18 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC). Recommended dilution: WB:1:500-2000, IHC:1:20-1:200. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin, type I cytoskeletal 18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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