Guinea pig anti-Human, Mouse Keratin K71, IRS1 Polyclonal Antibody
Keratin K71, IRS1 (Inner Root Sheath 1)
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
SerumSerum
Synonyms
Keratin K71; IRS1; Polyclonal Antibody; IRS1 (Inner Root Sheath 1); Anti -Keratin K71; anti-Keratin K71; IRS1 antibody
Host
Guinea pig
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human keratin K71 (57.3kD polypeptide; calculated from sequence data). The antiserum stains specifically the Henle, Huxley and cuticle layer of the inner root sheath (irs) of the hair follicle. Although there is a considerable sequence homology of keratin K71 to keratins K5 and K6, no crossreactivity was observed with Western Blot and immunofluorescence microscopy.
Purity/Purification
Serum
Serum
Serum
Form/Format
Supplied as a liquid, 0.09% sodium azide.
Applicable Applications for anti-Keratin K71, IRS1 antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunohistochemistry and Western Blot.
Dilution: Immunohistochemistry (Frozen): 1:100-1:200. Incubation time: 1 hour at RT.
Western Blot: 1:2000 using ECL.
Dilution: Immunohistochemistry (Frozen): 1:100-1:200. Incubation time: 1 hour at RT.
Western Blot: 1:2000 using ECL.
Immunogen
Synthetic peptide corresponding to human keratin K71, C-GGEGRSRGSANDYKDT (formerly also designated keratin K6irs1), conjugated to KLH.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-Keratin K71, IRS1 antibody
Insulin Receptor Substrate (IRS-1) is one of the major substrates of Insulin Receptor Kinase. IRS-1 contains multiple tyrosine phosphorylation motifs that serve as docking sites for SH2 domain-containing proteins (Grb2, p85, SHP-2, Fyn and others) and which mediate the metabolic and growth promoting functions of insulin. IRS-1 also contains over 30 potential Ser/Thr phosphorylation sites for kinases, such as PKA, PKC and MAPK. The phosphorylation of Ser 612 and Ser636/639 are mediated by PKA and mTOR pathways, respectively, resulting in an inhibition of insulin signaling in the cell and suggesting a potential mechanism for insulin resistance in some models of obesity. Once phosphorylated by insulin receptor, IRS-1 recruits the downstream components that mediate the insulin signaling. IRS-1 protein contains a conserved pleckstrin homology (PH) domain located at its amino terminus, adjacent to a phospho-tyrosine binding (PTB) domain.
Product Categories/Family for anti-Keratin K71, IRS1 antibody
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Product Notes
The Keratin K71, IRS1 (Catalog #AAA616368) is an Antibody produced from Guinea pig and is intended for research purposes only. The product is available for immediate purchase. The Keratin K71, IRS1 (Inner Root Sheath 1) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin K71, IRS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Immunohistochemistry and Western Blot. Dilution: Immunohistochemistry (Frozen): 1:100-1:200. Incubation time: 1 hour at RT. Western Blot: 1:2000 using ECL. Researchers should empirically determine the suitability of the Keratin K71, IRS1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin K71, IRS1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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