Western Blot (WB)
(Western BlotSample: Recombinant KRT25, Mouse.)
Rabbit anti-Mouse Keratin 25 Polyclonal Antibody | anti-KRT25 antibody
Polyclonal Antibody to Keratin 25 (KRT25)
Gene Names
Krt25; Ka38; Krt25a; mIRSa1; 4631426H08Rik
Reactivity
Mouse
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation
Purity
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Synonyms
Keratin 25; Polyclonal Antibody; Polyclonal Antibody to Keratin 25 (KRT25); anti-KRT25 antibody
Host
Rabbit
Reactivity
Mouse
Clonality
Polyclonal
Purity/Purification
Antigen-specific affinity chromatography followed by Protein A affinity chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
Concentration
0.66mg/ml (varies by lot)
Applicable Applications for anti-KRT25 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP)
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Polyclonal antibody preparation
Cross Reactivity
Mouse
Traits
Liquid
Immunogen
Recombinant KRT25 (Met1~Asn446) expressed in E.coli (MBS2031827)
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Avoid repeated freeze/thaw cycles.
Store at 4°C for frequent use.
Aliquot and store at -20°C for 24 months.
Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western BlotSample: Recombinant KRT25, Mouse.)
Western Blot (WB)
(Western BlotSample: Recombinant KRT25, Mouse.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
keratin, type I cytoskeletal 25
NCBI Official Synonym Full Names
keratin 25
NCBI Official Symbol
Krt25
NCBI Official Synonym Symbols
Ka38; Krt25a; mIRSa1; 4631426H08Rik
NCBI Protein Information
keratin, type I cytoskeletal 25
UniProt Protein Name
Keratin, type I cytoskeletal 25
Protein Family
UniProt Gene Name
Krt25
UniProt Synonym Gene Names
CK-25; K25; mIRSa1
Uniprot Description
KRT25: Essential for the proper assembly of type I and type II keratin protein complexes and formation of keratin intermediate filaments in the inner root sheath (irs). Belongs to the intermediate filament family.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 11|11 D
Cellular Component: cytoplasm; intermediate filament
Molecular Function: protein binding; protein heterodimerization activity; structural molecule activity
Biological Process: aging; cytoskeleton organization; hair cycle; hair follicle morphogenesis; intermediate filament organization
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Product Notes
The KRT25 krt25 (Catalog #AAA2027827) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Keratin 25 (KRT25) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 25 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC), Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the KRT25 krt25 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Keratin 25, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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