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Western Blot (WB)
(Western Blot: Sample: Lane1: Human MCF-7 Cells; Lane2: Human SGC7901 Cells; Lane3: Human Hela Cells.)
Rabbit Keratin 18 Polyclonal Antibody | anti-KRT18 antibody
Polyclonal Antibody to Keratin 18 (KRT18)
Gene Names
Krt18; Krt1-18
Reactivity
Rat
Applications
Western Blot, Immunocytochemistry, Immunohistochemistry, ELISA
Purity
Affinity Chromatography
Synonyms
Keratin 18; Polyclonal Antibody; Polyclonal Antibody to Keratin 18 (KRT18); anti-KRT18 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KRT18. It has beenselected for its ability to recognize KRT18 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Liquid
Concentration
0.31mg/ml (varies by lot)
Sequence
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EKETMQDLN DRLASYLDKV KNLETENRRL ESKIREYLEK RGPQGVRDWG HYFKTIEDLR AQIFANSVDN ARIVLQIDNA RLAADDFRVK YETELAMRQS VESDIHGLRK VVDDTNITRL QLETEIEALK EELLFMKKNH EEEVQGLEAQ IASSGLTVEV DAPKSQDLSK IMADIRAQYE QLAQKNREEL DKYWSQQIEE STTVVTTKSA EIRDAETTLL ELRRTLQTLE IDLDSMKNQN INLENNLGEV EARYRVQMEQ LNGVLLHLES ELAQTRAEGQ RQTQEYEALL NIKVKLEAEI ATYRRLLEDG
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EKETMQDLN DRLASYLDKV KNLETENRRL ESKIREYLEK RGPQGVRDWG HYFKTIEDLR AQIFANSVDN ARIVLQIDNA RLAADDFRVK YETELAMRQS VESDIHGLRK VVDDTNITRL QLETEIEALK EELLFMKKNH EEEVQGLEAQ IASSGLTVEV DAPKSQDLSK IMADIRAQYE QLAQKNREEL DKYWSQQIEE STTVVTTKSA EIRDAETTLL ELRRTLQTLE IDLDSMKNQN INLENNLGEV EARYRVQMEQ LNGVLLHLES ELAQTRAEGQ RQTQEYEALL NIKVKLEAEI ATYRRLLEDG
Applicable Applications for anti-KRT18 antibody
Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA)
Application Notes
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
Fragment
KRT18 (Glu72~Gly380)
Organism Species
Rattus norvegicus (Rat)
Cross Reactivity
Human, Rat
Conjugate
No Conjugate
Immunogen
Recombinant KRT18 (Glu72~Gly380) expressed in E Coli.
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2052888
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Lane1: Human MCF-7 Cells; Lane2: Human SGC7901 Cells; Lane3: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Lane1: Human MCF-7 Cells; Lane2: Human SGC7901 Cells; Lane3: Human Hela Cells.)
Western Blot (WB)
(Western Blot: Sample: Recombinant KRT18, Rat.)
Western Blot (WB)
(Western Blot: Sample: Recombinant KRT18, Rat.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Stomach Tissue)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue.)
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Intestine Tissue.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
47,761 Da
NCBI Official Full Name
keratin, type I cytoskeletal 18
NCBI Official Synonym Full Names
keratin 18
NCBI Official Symbol
Krt18
NCBI Official Synonym Symbols
Krt1-18
NCBI Protein Information
keratin, type I cytoskeletal 18
UniProt Protein Name
Keratin, type I cytoskeletal 18
Protein Family
UniProt Gene Name
Krt18
UniProt Synonym Gene Names
CK-18; K18
NCBI Description
involved in testicular differentiation [RGD, Feb 2006]
Uniprot Description
K18: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Keratin 18 and its filament partner keratin 8 are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Defects are a cause of cryptogenic cirrhosis.
Protein type: Cytoskeletal; Nucleolus
Chromosomal Location of Human Ortholog: 7q36
Cellular Component: cell-cell adherens junction; cytoplasm; cytosol; intermediate filament; keratin filament; microtubule organizing center; nuclear matrix; nucleolus
Molecular Function: RNA binding; structural molecule activity
Biological Process: Golgi to plasma membrane CFTR protein transport; intermediate filament cytoskeleton organization and biogenesis; negative regulation of apoptosis; tumor necrosis factor-mediated signaling pathway
Research Articles on KRT18
Similar Products
Product Notes
The KRT18 krt18 (Catalog #AAA2027331) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Keratin 18 (KRT18) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 18 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA). Western blotting: 0.5-2ug/ml Immunocytochemistry in formalin fixed cells: 5-20ug/ml Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml Immunohistochemistry in paraffin section: 5-20ug/ml Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the KRT18 krt18 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGS- EKETMQDLN DRLASYLDKV KNLETENRRL ESKIREYLEK RGPQGVRDWG HYFKTIEDLR AQIFANSVDN ARIVLQIDNA RLAADDFRVK YETELAMRQS VESDIHGLRK VVDDTNITRL QLETEIEALK EELLFMKKNH EEEVQGLEAQ IASSGLTVEV DAPKSQDLSK IMADIRAQYE QLAQKNREEL DKYWSQQIEE STTVVTTKSA EIRDAETTLL ELRRTLQTLE IDLDSMKNQN INLENNLGEV EARYRVQMEQ LNGVLLHLES ELAQTRAEGQ RQTQEYEALL NIKVKLEAEI ATYRRLLEDG. It is sometimes possible for the material contained within the vial of "Keratin 18, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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