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Western Blot (WB)
(Western Blot: Sample: Rat Skin lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT15 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Rabbit Keratin 15 (KRT15) Polyclonal Antibody | anti-KRT15 antibody
Polyclonal Antibody to Keratin 15 (KRT15)
Gene Names
Krt15; Ka15
Reactivity
Rat
Applications
Immunocytochemistry, Immunohistochemistry, ELISA, Western Blot
Purity
Affinity Chromatography
Synonyms
Keratin 15 (KRT15); Polyclonal Antibody; Polyclonal Antibody to Keratin 15 (KRT15); anti-KRT15 antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Specificity
The antibody is a rabbit polyclonal antibody raised against KRT15. It has been selected for its ability to recognize KRT15 in immunohistochemical staining andwestern blotting.
Purity/Purification
Affinity Chromatography
Form/Format
Supplied as solution form in PBS, pH7.4, containing 0.02% NaN3,50% glycerol.
Concentration
500ug/mL (varies by lot)
Sequence
Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSGSEF-EKVTMQN LNDRLASYLD KVRALEEANT ELEVKIRDWY QKQSPASPDR DYSHYFKTME EIRDKILAAT IDNSRVILEI DNARLAADDF RLKYENELAL RQGVEADING LRRVLDELTL ARTDLEMQIE QLNEELAYLK KNHEEEMKEF SSQLAGQVNV EMDAAPGVDL TRMLAEMREQ YEAIAEKNRR DVEAWFFSKT EELNKEVASN TEMIQTSKTE ITDLRRTLQG LEIELQSQLS MKAGLENSLA EVECRYATQL QQIQGLITGL ETQLSELRCE MEAQNQEYNM LLDIKTRLEQ EISTYRNLLE GQ
MGHHHHHHSGSEF-EKVTMQN LNDRLASYLD KVRALEEANT ELEVKIRDWY QKQSPASPDR DYSHYFKTME EIRDKILAAT IDNSRVILEI DNARLAADDF RLKYENELAL RQGVEADING LRRVLDELTL ARTDLEMQIE QLNEELAYLK KNHEEEMKEF SSQLAGQVNV EMDAAPGVDL TRMLAEMREQ YEAIAEKNRR DVEAWFFSKT EELNKEVASN TEMIQTSKTE ITDLRRTLQG LEIELQSQLS MKAGLENSLA EVECRYATQL QQIQGLITGL ETQLSELRCE MEAQNQEYNM LLDIKTRLEQ EISTYRNLLE GQ
Sequence Length
456
Applicable Applications for anti-KRT15 antibody
Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB)
Application Notes
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Immunogen
Recombinant KRT15 (Glu94~Gln402) expressed in E.coli.
Cross Reactivity
Human, Mouse, Rat
Conjugated Antibody
The APC conjugated antibody version of this item is also available as catalog #MBS2043229
Preparation and Storage
Store at 4 degree C for frequent use. Stored at -20 degree C to -80 degree C in a manual defrost freezer for one year without detectable loss of activity. Avoid repeated freeze-thaw cycles.
Western Blot (WB)
(Western Blot: Sample: Rat Skin lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT15 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Rat Skin lysate; Primary Ab: 1ug/ml Rabbit Anti-Rat KRT15 Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Western Blot (WB)
(Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded testis tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin- embedded testis tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue))
Immunohistochemistry (IHC)
(DAB staining on IHC-P; Samples: Rat Testis Tissue))
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
48,870 Da
NCBI Official Full Name
keratin, type I cytoskeletal 15
NCBI Official Synonym Full Names
keratin 15
NCBI Official Symbol
Krt15
NCBI Official Synonym Symbols
Ka15
NCBI Protein Information
keratin, type I cytoskeletal 15
UniProt Protein Name
Keratin, type I cytoskeletal 15
Protein Family
UniProt Gene Name
Krt15
UniProt Synonym Gene Names
CK-15; K15
NCBI Description
member of the keratin gene family; type I keratins are acidic proteins that heterodimerize with type II keratins in the formation of hair and nails [RGD, Feb 2006]
Uniprot Description
K15: a type I cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. There are two types of cytoskeletal and microfibrillar keratin: type I (acidic; 40-55 kDa) [K9 to K20] and type II (neutral to basic; 56-70 kDa) [K1 to K8]. Both a basic and an acidic keratin are required for filament assembly.
Protein type: Cytoskeletal
Chromosomal Location of Human Ortholog: 10q31
Cellular Component: intermediate filament; keratin filament; nucleus
Molecular Function: structural constituent of cytoskeleton
Biological Process: cytoskeleton organization; intermediate filament-based process
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Product Notes
The KRT15 krt15 (Catalog #AAA2002330) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Keratin 15 (KRT15) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Keratin 15 (KRT15) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the KRT15 krt15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with N-terminal His-Tag and its sequence is listed below. MGHHHHHHSG SEF-EKVTMQ N LNDRLASYLD KVRALEEANT ELEVKIRDWY QKQSPASPDR DYSHYFKTME EIRDKILAAT IDNSRVILEI DNARLAADDF RLKYENELAL RQGVEADING LRRVLDELTL ARTDLEMQIE QLNEELAYLK KNHEEEMKEF SSQLAGQVNV EMDAAPGVDL TRMLAEMREQ YEAIAEKNRR DVEAWFFSKT EELNKEVASN TEMIQTSKTE ITDLRRTLQG LEIELQSQLS MKAGLENSLA EVECRYATQL QQIQGLITGL ETQLSELRCE MEAQNQEYNM LLDIKTRLEQ EISTYRNLLE GQ. It is sometimes possible for the material contained within the vial of "Keratin 15 (KRT15), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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