Rabbit anti-Human Keratin 12/KRT12 Polyclonal Antibody | anti-KRT12 antibody

Anti-Keratin 12/KRT12 Antibody, Rabbit Polyclonal

Cat. Num. AAA8102039

• Gene Names: KRT12; K12
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Keratin 12/KRT12; Polyclonal Antibody; Anti-Keratin 12/KRT12 Antibody; Rabbit Polyclonal; Keratin 12/KRT12 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-K12 Antibody; anti-KRT12 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human Keratin 12/KRT12
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; PBS, pH7.0 with 0.03% Proclin300

• Applicable Applications for anti-KRT12 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:200-1:1000
• Immunogen: A synthetic peptide corresponding to the center region of the Human Keratin 12/KRT12
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the center region of the Human Keratin 12/KRT12, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human KRT12 in human colon carcinoma with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Immunohistochemistry (IHC)

(Immunochemical staining of human KRT12 in human duodenum with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

Related Product Information for anti-KRT12 antibody

Meesmann corneal dystrophy (MECD) is an autosomal dominant disorder affecting the corneal epithelium. It is caused by heterozygous mutations in KRT3 or KRT12 gene. Mutation of KRT12 gene in exon 1, T419A and L132H is the molecular genetic change leading to the occurrence of Meesmann's corneal dystrophy in this Chinese family.

NCBI and Uniprot Product Information

• NCBI GI #: 4557699
• NCBI GeneID: 3859
• NCBI Accession #: NP_000214.1
• NCBI GenBank Nucleotide #: NM_000223.3
• UniProt Accession #: Q99456; B2R9E0
• Molecular Weight: 53,511 Da
• NCBI Official Full Name: keratin, type I cytoskeletal 12
• NCBI Official Synonym Full Names: keratin 12
• NCBI Official Symbol: KRT12
• NCBI Official Synonym Symbols: K12
• NCBI Protein Information: keratin, type I cytoskeletal 12; CK-12; keratin-12; cytokeratin-12
• UniProt Protein Name: Keratin, type I cytoskeletal 12
• Protein Family: Keratin
• UniProt Gene Name: KRT12
• UniProt Synonym Gene Names: CK-12; K12
• UniProt Entry Name: K1C12_HUMAN

Uniprot Description

Function: May play a unique role in maintaining the normal corneal epithelial function. Together with KRT3, essential for the maintenance of corneal epithelium integrity

By similarity.

Subunit structure: Heterotetramer of two type I and two type II keratins. Keratin-3 associates with keratin-12.

Tissue specificity: Cornea specific. Ref.1

Involvement in disease: Corneal dystrophy, Meesmann (MECD) [MIM:122100]: An autosomal dominant corneal disease characterized by fragility of the anterior corneal epithelium. Patients are usually asymptomatic until adulthood when rupture of the corneal microcysts may cause erosions, producing clinical symptoms such as photophobia, contact lens intolerance and intermittent diminution of visual acuity. Rarely, subepithelial scarring causes irregular corneal astigmatism and permanent visual impairment. Histological examination shows a disorganized and thickened epithelium with widespread cytoplasmic vacuolation and numerous small, round, debris-laden intraepithelial cysts.Note: The disease is caused by mutations affecting the gene represented in this entry. Ref.2 Ref.3 Ref.8 Ref.9 Ref.10 Ref.11 Ref.12 Ref.13

Miscellaneous: There are two types of cytoskeletal and microfibrillar keratin: I (acidic; 40-55 kDa) and II (neutral to basic; 56-70 kDa).

Sequence similarities: Belongs to the intermediate filament family.