Western Blot (WB)
((0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Goat KEO4/ERLIN1 Polyclonal Antibody | anti-ERLIN1 antibody
Goat anti-KEO4/ERLIN1 Antibody
Gene Names
ERLIN1; KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69
Reactivity
Tested: Human; Expected from sequence similarity: Human
Applications
ELISA, Western Blot
Purity
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Synonyms
KEO4/ERLIN1; Polyclonal Antibody; Goat anti-KEO4/ERLIN1 Antibody; ERLIN1; ER lipid raft associated 1; C10orf69; Erlin-1; KE04; KEO4; SPFH1; Band_7 23-211 Keo4 (Interim) similar to C. elegans protein C42C1.9; OTTHUMP00000059425; SPFH domain family; member 1; ERLIN1 antibody; ER lipid raft associated 1 antibody; C10orf69 antibody; Erlin-1 antibody; KE04 antibody; KEO4 antibody; SPFH1 antibody; Band_7 23-211 Keo4 (Interim) similar to C. elegans protein C42C1.9 antibody; OTTHUMP00000059425 antibody; member 1 antibody; KEO4 / ERLIN1; anti-ERLIN1 antibody
Host
Goat
Reactivity
Tested: Human; Expected from sequence similarity: Human
Clonality
Polyclonal
Purity/Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Form/Format
Supplied at 0.5 mg/ml in Tris saline, 0. 02% sodium azide, pH7.3 with 0.5% bovine serum albumin.
Concentration
100ug specific antibody in 200ul (varies by lot)
Sequence
EALEPSGENVIQNKE
Sequence Length
348
Applicable Applications for anti-ERLIN1 antibody
Peptide ELISA (EIA), Western Blot (WB)
Application Notes
Peptide ELISA: Antibody detection limit dilution 1: 8000.
Western Blot: Approx 38kDa band observed in Human Placenta lysates (calculated MW of 39.2kDa according to NP_006450.2). Recommended concentration: 0.3-1ug/ml.
Western Blot: Approx 38kDa band observed in Human Placenta lysates (calculated MW of 39.2kDa according to NP_006450.2). Recommended concentration: 0.3-1ug/ml.
Immunogen
Peptide with sequence C-EALEPSGENVIQNKE, from the C Terminus of the protein sequence according to NP_006450.2.
Epitope
C Terminus
Note
Reported variants represent identical protein: NP_006450.2, NP_001094096.1
Preparation and Storage
Aliquot and store at -20 degree C. Minimize freezing and thawing.
Western Blot (WB)
((0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
Western Blot (WB)
((0.5ug/ml) staining of Human Placenta lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
38,926 Da
NCBI Official Full Name
erlin-1
NCBI Official Synonym Full Names
ER lipid raft associated 1
NCBI Official Symbol
ERLIN1
NCBI Official Synonym Symbols
KE04; KEO4; SPFH1; SPG62; Erlin-1; C10orf69
NCBI Protein Information
erlin-1
UniProt Protein Name
Erlin-1
Protein Family
UniProt Gene Name
ERLIN1
UniProt Synonym Gene Names
C10orf69; KE04; KEO4; SPFH1; SPFH domain-containing protein 1
UniProt Entry Name
ERLN1_HUMAN
Uniprot Description
SPFH1: Component of the ERLIN1/ERLIN2 complex which mediates the endoplasmic reticulum-associated degradation (ERAD) of inositol 1,4,5-trisphosphate receptors (IP3Rs). Belongs to the band 7/mec-2 family. Forms a heteromeric complex with ERLIN2.
Protein type: Membrane protein, integral
Chromosomal Location of Human Ortholog: 10q24.31
Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; protein complex
Molecular Function: cholesterol binding; protein binding
Biological Process: cholesterol metabolic process; ER-associated protein catabolic process; negative regulation of cholesterol biosynthetic process; negative regulation of fatty acid biosynthetic process; SREBP-mediated signaling pathway
Research Articles on ERLIN1
Similar Products
Product Notes
The ERLIN1 erlin1 (Catalog #AAA422090) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Goat anti-KEO4/ERLIN1 Antibody reacts with Tested: Human; Expected from sequence similarity: Human and may cross-react with other species as described in the data sheet. AAA Biotech's KEO4/ERLIN1 can be used in a range of immunoassay formats including, but not limited to, Peptide ELISA (EIA), Western Blot (WB). Peptide ELISA: Antibody detection limit dilution 1: 8000. Western Blot: Approx 38kDa band observed in Human Placenta lysates (calculated MW of 39.2kDa according to NP_006450.2). Recommended concentration: 0.3-1ug/ml. Researchers should empirically determine the suitability of the ERLIN1 erlin1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: EALEPSGENV IQNKE. It is sometimes possible for the material contained within the vial of "KEO4/ERLIN1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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