Mouse anti-Human MAPK8 / JNK1 / JNK Monoclonal Antibody | anti-MAPK8 antibody

Mouse Monoclonal [clone 1A2] (IgG2a,k) to Human MAPK8 / JNK1 / JNK

Cat. Num. AAA245550

• Gene Names: MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: MAPK8 / JNK1 / JNK; Monoclonal Antibody; Mouse Monoclonal [clone 1A2] (IgG2a; k) to Human MAPK8 / JNK1 / JNK; Anti-MAPK8 / JNK1 / JNK Antibody (clone 1A2) IHC-plus; MAPK8; JNK; JNK1; JNK1A2; JNK-46; MAP kinase 8; PRKM8; SAPK1; SAPK1c; MAPK 8; C-Jun N-terminal kinase 1; JNK21B1/2; JUN N-terminal kinase; Human MAPK8; anti-MAPK8 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1A2
• Specificity: Human MAPK8 / JNK1
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Sequence Length: 427

• Applicable Applications for anti-MAPK8 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB; Usage: Western Blot: Tissue lysate, Cell lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: Recombinant protein
• Immunogen Type: Recombinant protein
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-MAPK8 / JNK1 antibody IHC of human small intestine. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Immunohistochemistry (IHC)

(Anti-MAPK8 / JNK1 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(MAPK8 monoclonal antibody, clone 1A2. Western blot of MAPK8 expression in A-431.)

Western Blot (WB)

(MAPK8 monoclonal antibody, clone 1A2. Western blot of MAPK8 expression in human kidney.)

Related Product Information for anti-MAPK8 antibody

JNK1, a MAPK type protein kinase, regulates cell apoptosis, differentiation and growth in response to growth factors, inflammatory cytokines and cellular stress. JNK1 is phosphorylated by MKK4/MAP2K4 and MKK7/MAP2K7. Activated JNK1 translocates to the nucleus where it phosphorylates transcription factors, including c-Jun, ATF2, ELK1, Jun-D and NFAT4. At least four alternatively spliced transcript variants encoding distinct isoforms, 384 and 427 amino acids, have been identified.

Product Categories/Family for anti-MAPK8 antibody

Family: Protein Kinase; Subfamily: MAPK

NCBI and Uniprot Product Information

• NCBI GI #: 20986523
• NCBI GeneID: 5599
• NCBI Accession #: NP_620637.1
• NCBI GenBank Nucleotide #: NM_139049.2
• UniProt Accession #: P45983; Q15709; Q15712; Q15713; Q308M2; B5BTZ5; B7ZLV4; D3DX88; D3DX92
• Molecular Weight: 35,333 Da
• NCBI Official Full Name: mitogen-activated protein kinase 8 isoform alpha2
• NCBI Official Synonym Full Names: mitogen-activated protein kinase 8
• NCBI Official Symbol: MAPK8
• NCBI Official Synonym Symbols: JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2
• NCBI Protein Information: mitogen-activated protein kinase 8; MAP kinase 8; JUN N-terminal kinase; c-Jun N-terminal kinase 1; stress-activated protein kinase 1; stress-activated protein kinase 1c; mitogen-activated protein kinase 8 isoform JNK1 beta2; mitogen-activated protein kinase 8 isoform JNK1 alpha1
• UniProt Protein Name: Mitogen-activated protein kinase 8
• UniProt Gene Name: MAPK8
• UniProt Synonym Gene Names: JNK1; PRKM8; SAPK1; SAPK1C; MAP kinase 8; MAPK 8; SAPK1c
• UniProt Entry Name: MK08_HUMAN

NCBI Description

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Five alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jun 2013]

Uniprot Description

JNK1: a protein kinase of the MAPK family that is potently activated by a variety of environmental stresses, including UV and gamma radiation, ceramides, pro-inflammatory cytokines and, in some instances, by growth factors and GPCR agonists. Substrates include a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2, thus regulating AP-1 transcriptional activity. In T- cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. Binds to at least four scaffolding proteins, JIP-1, -2, -3 and -4. Activity increased in obesity. Inhibition or mouse knockout increases insulin sensitivity. Part of NFB pathway involved in inflammation and cancer, and signals downstream of Ras, though possibly as an apoptotic negative regulator of growth. Four alternatively-spliced isoforms have been described.

Protein type: Protein kinase, CMGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.24; CMGC group; MAPK family; MAPK/JNK subfamily; JNK subfamily

Chromosomal Location of Human Ortholog: 10q11.22

Cellular Component: nucleoplasm; mitochondrion; nucleus; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; histone deacetylase regulator activity; enzyme binding; histone deacetylase binding; JUN kinase activity; ATP binding

Biological Process: nerve growth factor receptor signaling pathway; positive regulation of apoptosis; apoptosis; rhythmic process; stress-activated MAPK cascade; toll-like receptor 3 signaling pathway; protein amino acid phosphorylation; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; regulation of protein localization; regulation of transcription factor activity; JNK cascade; response to stress; negative regulation of protein binding; toll-like receptor 4 signaling pathway; response to UV; regulation of histone deacetylation; ossification; MyD88-independent toll-like receptor signaling pathway; peptidyl-threonine phosphorylation; regulation of circadian rhythm; JUN phosphorylation; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; peptidyl-serine phosphorylation; response to cadmium ion; toll-like receptor signaling pathway; innate immune response; toll-like receptor 9 signaling pathway; negative regulation of apoptosis