Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Kdm6b antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Rabbit anti-Rat Kdm6b Polyclonal Antibody | anti-Kdm6b antibody
Kdm6b Polyclonal Antibody
Gene Names
Kdm6b; Jmjd3; BC038313; mKIAA0346; 1700064E03Rik
Reactivity
Rat
Applications
Western Blot
Purity
Affinity Purification
Synonyms
Kdm6b; Polyclonal Antibody; Kdm6b Polyclonal Antibody; JMJD3; anti-Kdm6b antibody
Host
Rabbit
Reactivity
Rat
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity Purification
Sequence
AQSVKPKINTEEKLPREKLNPPTPSIYLESKRDAFSPVLLQFCTDPRNPITVIRGLAGSLRLNLGLFSTKTLVEASGEHTVEVRTQVQQPSDENWDLTGTRQIWPCESSRSHTTIAKYAQYQASSFQESLQEERESEDEESEEPDSTTGTSPSSAPDPKNHHIIKFGTNIDLSDAKRWKPQLQELLKLPA
Sequence Length
671
Applicable Applications for anti-Kdm6b antibody
Western Blot (WB)
Application Notes
WB: 1:500 - 1:2000
Immunogen
Recombinant protein of mouse Kdm6b
Immunogen Species
Mouse
Storage Buffer
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Cellular Location
Nucleus
Positive Samples
Rat uterus
Preparation and Storage
Store at -20 degree C. Avoid freeze / thaw cycles.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Kdm6b antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using Kdm6b antibody at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
Product Categories/Family for anti-Kdm6b antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
Calculated: 176kDa
Observed: 200kDa
Observed: 200kDa
NCBI Official Full Name
KDM6B
NCBI Official Synonym Full Names
KDM1 lysine (K)-specific demethylase 6B
NCBI Official Symbol
Kdm6b
NCBI Official Synonym Symbols
Jmjd3; BC038313; mKIAA0346; 1700064E03Rik
NCBI Protein Information
lysine-specific demethylase 6B
UniProt Protein Name
Lysine-specific demethylase 6B
Protein Family
UniProt Gene Name
Kdm6b
UniProt Synonym Gene Names
Jmjd3; Kiaa0346
Uniprot Description
Histone demethylase that specifically demethylates 'Lys-27' of histone H3, thereby playing a central role in histone code. Demethylates trimethylated and dimethylated H3 'Lys-27'. Plays a central role in regulation of posterior development, by regulating HOX gene expression. Involved in inflammatory response by participating in macrophage differentiation in case of inflammation by regulating gene expression and macrophage differentiation (PubMed:17825402). Plays a demethylase-independent role in chromatin remodeling to regulate T-box family member-dependent gene expression by acting as a link between T-box factors and the SMARCA4-containing SWI/SNF remodeling complex (PubMed:21095589).
Research Articles on Kdm6b
Similar Products
Product Notes
The Kdm6b kdm6b (Catalog #AAA9132966) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Kdm6b Polyclonal Antibody reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Kdm6b can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). WB: 1:500 - 1:2000. Researchers should empirically determine the suitability of the Kdm6b kdm6b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: AQSVKPKINT EEKLPREKLN PPTPSIYLES KRDAFSPVLL QFCTDPRNPI TVIRGLAGSL RLNLGLFSTK TLVEASGEHT VEVRTQVQQP SDENWDLTGT RQIWPCESSR SHTTIAKYAQ YQASSFQESL QEERESEDEE SEEPDSTTGT SPSSAPDPKN HHIIKFGTNI DLSDAKRWKP QLQELLKLPA. It is sometimes possible for the material contained within the vial of "Kdm6b, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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