Rabbit anti-Human KDELR2 Polyclonal Antibody | anti-KDELR2 antibody

KDELR2, CT (KDELR2, ERD2.2, ER lumen protein retaining receptor 2, ERD2-like protein 1, KDEL endoplasmic reticulum protein retention receptor 2) (AP)

Cat. Num. AAA6312973

• Gene Names: KDELR2; ELP-1; ERD2.2
• Reactivity: Human
• Applications: Immunohistochemistry, ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KDELR2; Polyclonal Antibody; CT (KDELR2; ERD2.2; ER lumen protein retaining receptor 2; ERD2-like protein 1; KDEL endoplasmic reticulum protein retention receptor 2) (AP); KDEL endoplasmic reticulum protein retention receptor 2; anti-KDELR2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase (AP).

• Applicable Applications for anti-KDELR2 antibody: Immunohistochemistry (IHC), ELISA (EIA), Western Blot (WB)
• Application Notes: IHC: 1:10-50; ELISA: 1:1,000; WB: 1:100-500; Applications are based on unconjugated antibody.
• Immunogen: KDELR2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 182-211 amino acids from the C-terminal region of human KDELR2.
• Conjugate: AP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(KDELR2 Antibody (C-term) western blot analysis in T47D cell line lysates (35ug/lane).This demonstrates the KDELR2 antibody detected the KDELR2 protein (arrow).)

Immunohistochemistry (IHC)

(KDELR2 Antibody (C-term) (AP14724b)immunohistochemistry analysis in formalin fixed and paraffin embedded human cerebellum tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of KDELR2 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

Related Product Information for anti-KDELR2 antibody

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. KDELR2 was the second member of the family to be identified, and it encodes a protein which is 83% identical to the KDELR1 gene product. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq].

Product Categories/Family for anti-KDELR2 antibody

Antibodies; Receptors

References

van der Vlies, D., et al. Biochem. J. 366 (PT 3), 825-830 (2002): Pelham, H.R. Cell Struct. Funct. 21(5):413-419(1996). Lewis, M.J., et al. J. Mol. Biol. 226(4):913-916(1992) Hsu, V.W., et al. Cell 69(4):625-635(1992).

NCBI and Uniprot Product Information

• NCBI GI #: 5803050
• NCBI GeneID: 11014
• NCBI Accession #: NP_006845.1; NP_001094073.1
• NCBI GenBank Nucleotide #: NM_006854.3
• UniProt Accession #: P33947; Q6IPC5; Q96E30; A4D2P4
• Molecular Weight: 21,242 Da
• NCBI Official Full Name: ER lumen protein-retaining receptor 2 isoform 1
• NCBI Official Synonym Full Names: KDEL endoplasmic reticulum protein retention receptor 2
• NCBI Official Symbol: KDELR2
• NCBI Official Synonym Symbols: ELP-1; ERD2.2
• NCBI Protein Information: ER lumen protein-retaining receptor 2
• UniProt Protein Name: ER lumen protein retaining receptor 2
• Protein Family: ER lumen protein-retaining receptor
• UniProt Gene Name: KDELR2
• UniProt Synonym Gene Names: ERD2.2; ELP-1
• UniProt Entry Name: ERD22_HUMAN

NCBI Description

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. KDELR2 was the second member of the family to be identified, and it encodes a protein which is 83% identical to the KDELR1 gene product. Alternative splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

KDELR2: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L. Belongs to the ERD2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 7p22.1

Cellular Component: Golgi apparatus; endoplasmic reticulum membrane; cis-Golgi network; endoplasmic reticulum; integral to membrane

Molecular Function: KDEL sequence binding

Biological Process: vesicle-mediated transport; intracellular protein transport; protein retention in ER