Rabbit anti-Human REST Polyclonal Antibody | anti-REST antibody

REST, ID (REST, NRSF, XBR, RE1-silencing transcription factor, Neural-restrictive silencer factor, X2 box repressor) (MaxLight 750)

Cat. Num. AAA6341343

• Gene Names: REST; WT6; XBR; NRSF
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: REST; Polyclonal Antibody; ID (REST; NRSF; XBR; RE1-silencing transcription factor; Neural-restrictive silencer factor; X2 box repressor) (MaxLight 750); X2 box repressor; anti-REST antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human REST.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight750.

• Applicable Applications for anti-REST antibody: Western Blot (WB)
• Application Notes: WB: 1:1000; Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 542-570 in the central region of human human REST, UniProt Accession #Q13127; NP_005603.3; NP_001180437.1.
• Conjugate: MaxLight750
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight750 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Testing Data

(All lanes: 040963 (1:2000) Lane 1: Hela whole cell lysate (20ug) Lane 2: Jurkat whole cell lysate (20ug) Lane 3: PC-3 whole cell lysate (20ug) Lane 4: Raji whole cell lysate (20ug) Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 122kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Testing Data

(All lanes: 040963 (1:2000) Lane 1: 293T/17 whole cell lysate (20ug) Lane 2: Jurkat whole cell lysate (20ug) Lane 3: Raji whole cell lysate (20ug). Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1:10000 dilution. Predicted band size: 122kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Product Categories/Family for anti-REST antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 301897974
• NCBI GeneID: 5978
• NCBI Accession #: NP_005603.3; NP_001180437.1
• NCBI GenBank Nucleotide #: NM_005612.4
• UniProt Accession #: Q13127; Q12956; Q12957; Q13134; Q59ER1; Q8IWI3; A2RUE0; B9EGJ0
• Molecular Weight: 119,083 Da
• NCBI Official Full Name: RE1-silencing transcription factor
• NCBI Official Synonym Full Names: RE1 silencing transcription factor
• NCBI Official Symbol: REST
• NCBI Official Synonym Symbols: WT6; XBR; NRSF
• NCBI Protein Information: RE1-silencing transcription factor
• UniProt Protein Name: RE1-silencing transcription factor
• Protein Family: Restin
• UniProt Gene Name: REST
• UniProt Synonym Gene Names: NRSF; XBR
• UniProt Entry Name: REST_HUMAN

NCBI Description

This gene encodes a transcriptional repressor that represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described [provided by RefSeq, Jul 2010]