Rabbit anti-Human KDELR1 Polyclonal Antibody | anti-KDELR1 antibody

KDELR1, CT (KDELR1, ERD2.1, ER lumen protein retaining receptor 1, KDEL endoplasmic reticulum protein retention receptor 1, Putative MAPK-activating protein PM23) (Azide free) (HRP)

Cat. Num. AAA6312966

• Gene Names: KDELR1; ERD2; HDEL; PM23; ERD2.1
• Reactivity: Human
• Applications: Western Blot, ELISA
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: KDELR1; Polyclonal Antibody; CT (KDELR1; ERD2.1; ER lumen protein retaining receptor 1; KDEL endoplasmic reticulum protein retention receptor 1; Putative MAPK-activating protein PM23) (Azide free) (HRP); Putative MAPK-activating protein PM23; anti-KDELR1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-KDELR1 antibody: Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:100-500; ELISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: KDELR1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 184-211 amino acids from the C-terminal region of human KDELR1.
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(KDELR1 Antibody (C-term) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the KDELR1 antibody detected the KDELR1 protein (arrow).)

Related Product Information for anti-KDELR1 antibody

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product.

Product Categories/Family for anti-KDELR1 antibody

Antibodies; Receptors

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007): Breuza, L., et al. J. Biol. Chem. 279(45):47242-47253(2004). Bard, F., et al. J. Biol. Chem. 278(47):46601-46606(2003). Yamamoto, K., et al. J. Biol. Chem. 278(36):34525-34532(2003). Matsuda, A., et al. Oncogene 22(21):3307-3318(2003).

NCBI and Uniprot Product Information

• NCBI GI #: 5803048
• NCBI GeneID: 10945
• NCBI Accession #: NP_006792.1
• NCBI GenBank Nucleotide #: NM_006801.2
• UniProt Accession #: P24390; Q54A39; Q8NBW7; B2R6N4
• Molecular Weight: 78KD
• NCBI Official Full Name: ER lumen protein-retaining receptor 1
• NCBI Official Synonym Full Names: KDEL endoplasmic reticulum protein retention receptor 1
• NCBI Official Symbol: KDELR1
• NCBI Official Synonym Symbols: ERD2; HDEL; PM23; ERD2.1
• NCBI Protein Information: ER lumen protein-retaining receptor 1
• UniProt Protein Name: ER lumen protein retaining receptor 1
• Protein Family: ER lumen protein-retaining receptor
• UniProt Gene Name: KDELR1
• UniProt Synonym Gene Names: ERD2.1
• UniProt Entry Name: ERD21_HUMAN

NCBI Description

Retention of resident soluble proteins in the lumen of the endoplasmic reticulum (ER) is achieved in both yeast and animal cells by their continual retrieval from the cis-Golgi, or a pre-Golgi compartment. Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually lys-asp-glu-leu (KDEL) in animal cells, and his-asp-glu-leu (HDEL) in S. cerevisiae. This process is mediated by a receptor that recognizes, and binds the tetrapeptide-containing protein, and returns it to the ER. In yeast, the sorting receptor encoded by a single gene, ERD2, which is a seven-transmembrane protein. Unlike yeast, several human homologs of the ERD2 gene, constituting the KDEL receptor gene family, have been described. The protein encoded by this gene was the first member of the family to be identified, and it encodes a protein structurally and functionally similar to the yeast ERD2 gene product. [provided by RefSeq, Jul 2008]

Uniprot Description

KDELR1: Required for the retention of luminal endoplasmic reticulum proteins. Determines the specificity of the luminal ER protein retention system. Also required for normal vesicular traffic through the Golgi. This receptor recognizes K-D-E-L. Belongs to the ERD2 family.

Protein type: Endoplasmic reticulum; Membrane protein, multi-pass; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: COPI coated vesicle membrane; endoplasmic reticulum membrane; ER-Golgi intermediate compartment membrane; cis-Golgi network; membrane; ER-Golgi intermediate compartment; integral to membrane

Molecular Function: KDEL sequence binding

Biological Process: vesicle-mediated transport; intracellular protein transport; protein retention in ER