Rabbit anti-Human KCC3 Polyclonal Antibody | anti-SLC12A6 antibody

KCC3 (K, Cl Cotransporter 3)

Cat. Num. AAA614570

• Gene Names: SLC12A6; KCC3; ACCPN; KCC3A; KCC3B; DKFZp434D2135
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Serum; Serum
• Synonyms: KCC3; Polyclonal Antibody; KCC3 (K; Cl Cotransporter 3); Anti -KCC3 (K; anti-SLC12A6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human KCC3 and KCC-3b spliced form. Species Sequence Homology: rat-89%, mouse-78% (KCC3b).
• Purity/Purification: Serum; Serum
• Form/Format: Supplied as a liquid in PBS, 0.05% sodium azide, before the addition of glycerol to 40%.

• Applicable Applications for anti-SLC12A6 antibody: ELISA (EL/EIA), Western Blot (WB)
• Application Notes: Suitable for use in ELISA and Western Blot.; Dilution: Western Blot: 1:250-1:500. Band seen at 95-110kD (brush border membrane).; ELISA: 1:5000-50,000. Control peptide can be used to coat ELISA plates at 1ug/ml.
• Immunogen: Synthetic peptide corresponding to 17aa sequence within the cytoplasmic, N-terminal domain of the human KCC3 (KLH).
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage, aliquot and store at -20 degree C. Aliquots are stable for at least 3 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-SLC12A6 antibody

Chloride is a critical component of all living cells. It is also the single most dominant diffusible anion inside of most cells-the others are mostly impermeable organic anions. Since cytoplasmic electroneutrality is maintained under normal physiological environment, changes in cellular chloride level is accompanied by total cell solute content. The cation chloride cotransporters (CCC) protein family is involved in the electroneutral movement of ions across the cell membrane.

Product Categories/Family for anti-SLC12A6 antibody

Antibodies; Abs to Ion Channel

NCBI and Uniprot Product Information

• NCBI GI #: 6693798
• NCBI GeneID: 9990
• UniProt Accession #: Q9UHW9; Q2VI00; Q7Z2E7; Q7Z4G5; Q8TDD4; Q9UFR2; Q9Y642; Q9Y665; Q3ZCQ6; Q5HYC7; Q6NSI7; A0AV76
• Molecular Weight: 127,617 Da
• NCBI Official Full Name: K-Cl cotransporter KCC3
• NCBI Official Synonym Full Names: solute carrier family 12 (potassium/chloride transporters), member 6
• NCBI Official Symbol: SLC12A6
• NCBI Official Synonym Symbols: KCC3; ACCPN; KCC3A; KCC3B; DKFZp434D2135
• NCBI Protein Information: solute carrier family 12 member 6; OTTHUMP00000159813; K-Cl cotransporter 3; potassium-chloride transporter-3a; potassium-chloride transporter-3b; potassium chloride cotransporter 3; potassium chloride cotransporter KCC3a-S3; electroneutral potassium-chloride cotransporter 3
• UniProt Protein Name: Solute carrier family 12 member 6
• Protein Family: Solute carrier family
• UniProt Gene Name: SLC12A6
• UniProt Synonym Gene Names: KCC3
• UniProt Entry Name: S12A6_HUMAN

NCBI Description

This gene is a member of the K-Cl cotransporter (KCC) family. K-Cl cotransporters are integral membrane proteins that lower intracellular chloride concentrations below the electrochemical equilibrium potential. The proteins encoded by this gene are activated by cell swelling induced by hypotonic conditions. Alternate splicing results in multiple transcript variants encoding different isoforms. Mutations in this gene are associated with agenesis of the corpus callosum with peripheral neuropathy. [provided by RefSeq]

Uniprot Description

KCC3: Mediates electroneutral potassium-chloride cotransport. May be activated by cell swelling. May contribute to cell volume homeostasis in single cells. Homomultimer and heteromultimer with other K-Cl cotransporters. Up-regulated by VEGF. Down-regulated by TNF. Highly expressed in heart, brain and kidney. Detected at lower levels in skeletal muscle, placenta, lung and pancreas. Detected in umbilical vein endothelial cells. Isoform 2 is more abundant in kidney. Isoform 5 is testis specific. Expressed in the proximal tubule of the kidney. Activated by N-ethylmaleimide (NEM). Inhibited by DIOA, bumetanide and furosemide. Inhibited by WNK3. Belongs to the SLC12A transporter family. 6 isoforms of the human protein are produced by alternative promoter.

Protein type: Transporter, SLC family; Membrane protein, integral; Transporter; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 15q13

Cellular Component: basolateral plasma membrane; integral to plasma membrane; plasma membrane; integral to membrane

Molecular Function: potassium ion transmembrane transporter activity; protein kinase binding; potassium:chloride symporter activity

Biological Process: potassium ion import; ion transport; angiogenesis; transmembrane transport; potassium ion transport

Disease: Agenesis Of The Corpus Callosum With Peripheral Neuropathy