Rabbit KATII / AADAT Polyclonal Antibody | anti-KATII antibody

Rabbit Polyclonal to Human KATII / AADAT

Cat. Num. AAA247462

• Gene Names: AADAT; KAT2; KATII
• Reactivity: Human; Predicted Reactivity: Gorilla, Gibbon, Monkey (at least 90% immunogen sequence identity)
• Applications: Immunohistochemistry
• Purity: Immunoaffinity Purified
• Synonyms: KATII / AADAT; Polyclonal Antibody; Rabbit Polyclonal to Human KATII / AADAT; Anti-KATII / AADAT Antibody (Internal) IHC-plus; AADAT; 2-aminoadipate transaminase; KAT2; KAT; AadAT; KATII; Kynurenine aminotransferase II; Aminoadipate aminotransferase; Human KATII; anti-KATII antibody

• Host: Rabbit
• Reactivity: Human; Predicted Reactivity: Gorilla, Gibbon, Monkey (at least 90% immunogen sequence identity)
• Clonality: Polyclonal
• Specificity: Human AADAT. BLAST analysis of the peptide immunogen showed no homology with other human proteins.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: PBS, 0.1% sodium azide.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 425

• Applicable Applications for anti-KATII antibody: Immunohistochemistry (IHC - Paraffin)
• Application Notes: IHC-P (5 ug/ml)
• Target Species: Human
• Immunogen Description: Synthetic 17 amino acid peptide from internal region of human AADAT. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon, Monkey (94%); Marmoset (88%); Panda, Pig (82%).
• Immunogen Type: Synthetic peptide
• Immunogen: KATII / AADAT antibody was raised against synthetic 17 amino acid peptide from internal region of human AADAT. Percent identity with other species by BLAST analysis: Human (100%); Gorilla, Gibbon, Monkey (94%); Marmoset (88%); Panda, Pig (82%).
• Antigen Modification: Internal
• Preparation and Storage: Store at 4 degree C for short term applications. For long term storage, aliquot and store at -20 degree C.

Immunohistochemistry (IHC)

(Anti-KATII / AADAT antibody IHC of human Colon, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-AADAT antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Immunohistochemistry (IHC)

(Anti-KATII / AADAT antibody IHC of human Lymph Node, Non-Hodgkins Lymphoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Related Product Information for anti-KATII antibody

Kynurenine aminotransferase catalyzes the irreversible transamination of the L-tryptophan metabolite L-kynurenine to form kynurenic acid. Kynurenic acid is an antagonist at the glycine site of NMDA receptors and increased levels of kynurenic acid are thought to exert a neuroprotective action in some pathological conditions.

NCBI and Uniprot Product Information

• NCBI GI #: 7705897
• NCBI GeneID: 51166
• NCBI Accession #: NP_057312.1
• NCBI GenBank Nucleotide #: NM_016228.3
• UniProt Accession #: Q8N5Z0; Q9UL02; B3KP84
• Molecular Weight: 47,822 Da
• NCBI Official Full Name: kynurenine/alpha-aminoadipate aminotransferase, mitochondrial isoform b
• NCBI Official Synonym Full Names: aminoadipate aminotransferase
• NCBI Official Symbol: AADAT
• NCBI Official Synonym Symbols: KAT2; KATII
• NCBI Protein Information: kynurenine/alpha-aminoadipate aminotransferase, mitochondrial; KAT/AadAT; 2-aminoadipate transaminase; kynurenine aminotransferase II; 2-aminoadipate aminotransferase; kynurenine--oxoglutarate transaminase 2; kynurenine--oxoglutarate transaminase II; kynurenine--oxoglutarate aminotransferase II; L kynurenine/alpha aminoadipate aminotransferase
• UniProt Protein Name: Kynurenine/alpha-aminoadipate aminotransferase, mitochondrial
• UniProt Gene Name: AADAT
• UniProt Synonym Gene Names: KAT2; KAT/AadAT; AadAT
• UniProt Entry Name: AADAT_HUMAN

NCBI Description

This gene encodes a protein that is highly similar to mouse and rat kynurenine aminotransferase II. The rat protein is a homodimer with two transaminase activities. One activity is the transamination of alpha-aminoadipic acid, a final step in the saccaropine pathway which is the major pathway for L-lysine catabolism. The other activity involves the transamination of kynurenine to produce kynurenine acid, the precursor of kynurenic acid which has neuroprotective properties. Several transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2013]

Uniprot Description

AADAT: Transaminase with broad substrate specificity. Has transaminase activity towards aminoadipate, kynurenine, methionine and glutamate. Shows activity also towards tryptophan, aspartate and hydroxykynurenine. Accepts a variety of oxo-acids as amino- group acceptors, with a preference for 2-oxoglutarate, 2- oxocaproic acid, phenylpyruvate and alpha-oxo-gamma-methiol butyric acid. Can also use glyoxylate as amino-group acceptor (in vitro). Belongs to the class-I pyridoxal-phosphate-dependent aminotransferase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - lysine biosynthesis; EC 2.6.1.7; EC 2.6.1.39; Transferase; Amino Acid Metabolism - tryptophan; Amino Acid Metabolism - lysine degradation; Mitochondrial

Chromosomal Location of Human Ortholog: 4q33

Cellular Component: mitochondrial matrix

Molecular Function: protein homodimerization activity; kynurenine-oxoglutarate transaminase activity; 2-aminoadipate transaminase activity; pyridoxal phosphate binding

Biological Process: glutamate metabolic process; tryptophan catabolic process to kynurenine; L-lysine catabolic process to acetyl-CoA via saccharopine; lysine catabolic process; biosynthetic process; tryptophan catabolic process; 2-oxoglutarate metabolic process