Rabbit anti-Human Kallikrein 6 Polyclonal Antibody | anti-KLK6 antibody

Kallikrein 6, ID (KLK6, PRSS18, PRSS9, Kallikrein-6, Neurosin, Protease M, SP59, Serine protease 18, Serine protease 9, Zyme) (MaxLight 405)

Cat. Num. AAA6312010

• Gene Names: KLK6; hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, Immunohistochemistry, Western Blot
• Purity: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Synonyms: Kallikrein 6; Polyclonal Antibody; ID (KLK6; PRSS18; PRSS9; Kallikrein-6; Neurosin; Protease M; SP59; Serine protease 18; Serine protease 9; Zyme) (MaxLight 405); KLK6; Zyme; anti-KLK6 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by saturated ammonium sulfate precipitation followed by dialysis against PBS.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight405.

• Applicable Applications for anti-KLK6 antibody: Flow Cytometry (FC/FACS), FLISA, Immunohistochemistry (IHC), Western Blot (WB)
• Application Notes: FC/FACS: 1:10-50; FLISA: 1:1,000; IHC: 1:10-50; WB: 1:50-100; Applications are based on unconjugated antibody.
• Immunogen: Kallikrein 6 Antibody antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 134~164 amino acids from the central region of human KLK6.
• Conjugate: MaxLight405
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight405 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western blot analysis of KLK6 (arrow) using rabbit polyclonal KLK6 Antibody (L141). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the KLK6 gene.)

Immunohistochemistry (IHC)

(Kallikrein 6(KLK6) Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of Kallikrein 6(KLK6) Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(Kallikrein 6 (KLK6) Antibody (Center) Antibody flow cytometric analysis of 293 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KLK6 antibody

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. The KLK6 enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease.

Product Categories/Family for anti-KLK6 antibody

Antibodies; Kallikrein

References

Christophi, G.P., et al., J. Neurochem. 91(6):1439-1449 (2004). Bayes, A., et al., Biol. Chem. 385(6):517-524 (2004). Pampalakis, G., et al., Biochem. Biophys. Res. Commun. 320(1):54-61 (2004). Ghosh, M.C., et al., Tumour Biol. 25(4):193-199 (2004). Sauter, E.R., et al., Int. J. Cancer 108(4):588-591 (2004).

NCBI and Uniprot Product Information

• NCBI GI #: 61744426
• NCBI GeneID: 5653
• NCBI Accession #: NP_001012983.1
• NCBI GenBank Nucleotide #: NM_001012965.1
• UniProt Accession #: Q92876; Q6H301; A6NJA1; A8MW09
• Molecular Weight: 26,856 Da
• NCBI Official Full Name: kallikrein-6 isoform B
• NCBI Official Synonym Full Names: kallikrein-related peptidase 6
• NCBI Official Symbol: KLK6
• NCBI Official Synonym Symbols: hK6; Bssp; Klk7; SP59; PRSS9; PRSS18
• NCBI Protein Information: kallikrein-6; zyme; neurosin; protease M; serine protease 9; serine protease 18
• UniProt Protein Name: Kallikrein-6
• Protein Family: Kallikrein
• UniProt Gene Name: KLK6
• UniProt Synonym Gene Names: PRSS18; PRSS9
• UniProt Entry Name: KLK6_HUMAN

NCBI Description

Kallikreins are a subgroup of serine proteases having diverse physiological functions. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers. This gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. The encoded enzyme is regulated by steroid hormones. In tissue culture, the enzyme has been found to generate amyloidogenic fragments from the amyloid precursor protein, suggesting a potential for involvement in Alzheimer's disease. Multiple alternatively spliced transcript variants that encode different isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

KLK6: Serine protease which exhibits a preference for Arg over Lys in the substrate P1 position and for Ser or Pro in the P2 position. Shows activity against amyloid precursor protein, myelin basic protein, gelatin, casein and extracellular matrix proteins such as fibronectin, laminin, vitronectin and collagen. Degrades alpha-synuclein and prevents its polymerization, indicating that it may be involved in the pathogenesis of Parkinson disease and other synucleinopathies. May be involved in regulation of axon outgrowth following spinal cord injury. Tumor cells treated with a neutralizing KLK6 antibody migrate less than control cells, suggesting a role in invasion and metastasis. Belongs to the peptidase S1 family. Kallikrein subfamily. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; EC 3.4.21.-; Secreted, signal peptide; Nucleolus; Secreted

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; microtubule cytoskeleton; extracellular space; intercellular bridge; nuclear membrane; mitochondrion; endoplasmic reticulum; cytoplasm; extracellular region; nucleolus

Molecular Function: protein binding; serine-type endopeptidase activity

Biological Process: myelination; collagen catabolic process; protein autoprocessing; central nervous system development; amyloid precursor protein metabolic process; tissue regeneration; positive regulation of G-protein coupled receptor protein signaling pathway; regulation of cell differentiation; response to wounding; hormone metabolic process; protein processing; proteolysis