Rabbit anti-Human K2C78 Polyclonal Antibody | anti-KRT78 antibody

K2C78 Antibody (Center)

Cat. Num. AAA9210191

• Gene Names: KRT78; K5B; K78; Kb40; CK-78
• Reactivity: Human
• Applications: Western Blot, ELISA, Flow Cytometry
• Purity: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Synonyms: K2C78; Polyclonal Antibody; K2C78 Antibody (Center); Keratin; type II cytoskeletal 78; Cytokeratin-78; CK-78; Keratin-5b; Keratin-78; K78; Type-II keratin Kb40; KRT78; K5B; KB40; anti-KRT78 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit Ig
• Specificity: This K2C78 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 176-203 amino acids from the Central region of human K2C78.
• Purity/Purification: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Positions: 176-203
• Sequence Length: 520

• Applicable Applications for anti-KRT78 antibody: Western Blot (WB), ELISA (EIA), Flow Cytometry (FC)
• Application Notes: WB~~1:1000; FC~~1:10~50
• Tissue Location: In non-keratinising esophageal and vaginal epithelium, strongly expressed in the basal and parabasal/lower suprabasal cell layers with considerably decreased expression in the mid/upper suprabasal layers (at protein level) (PubMed:26340985). A similar gradient from basal to lower suprabasal layers is seen in the partially keratinised dorsal tongue epithelium, in the scalp and in the plantar epidermis (at protein level) (PubMed:26340985). Extension of expression into the suprabasal compartments is distinctly more pronounced in non-keratinising epithelia than in keratinising epithelia and epidermis (at protein level) (PubMed:26340985). In scalp sections, present in the interfollicular epidermis and infundibulum including the entire outer root sheath of the hair follicles and also in the sebocytes (at protein level) (PubMed:26340985). In sweat glands, expressed in peripheral and luminal cells of the lower duct and in peripheral cells of the middle/upper duct with no expression observed in luminal cells (at protein level) (PubMed:26340985). In embryos at the 14th week of pregnancy, detected in basal and parabasal layers but is absent from the uppermost epidermal layer (at protein level) (PubMed:26340985). Expressed in tongue epithelium (PubMed:15737194) gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified.
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of K2C78 Antibody (Center)(MBS9210191) in Hela cell line lysates (35ug/lane). K2C78 (arrow) was detected using the purified Pab.)

Flow Cytometry (FC/FACS)

(K2C78 Antibody (Center) (MBS9210191) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Related Product Information for anti-KRT78 antibody

This gene is a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified.

Product Categories/Family for anti-KRT78 antibody

Signal Transduction

References

Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007)
Schweizer, J., et al. J. Cell Biol. 174(2):169-174(2006)
Rogers, M.A., et al. J. Invest. Dermatol. 124(3):536-544(2005)
Hesse, M., et al. Eur. J. Cell Biol. 83(1):19-26(2004)
Coulombe, P.A., et al. Curr. Opin. Cell Biol. 14(1):110-122(2002)

NCBI and Uniprot Product Information

• NCBI GI #: 89357932
• NCBI GeneID: 196374
• NCBI Accession #: NP_775487.2
• NCBI GenBank Nucleotide #: NM_173352.3
• UniProt Accession #: Q8N1N4; Q5HYM7; Q7RTT2; A8K4D6
• Molecular Weight: 56866
• NCBI Official Full Name: keratin, type II cytoskeletal 78 isoform 1
• NCBI Official Synonym Full Names: keratin 78, type II
• NCBI Official Symbol: KRT78
• NCBI Official Synonym Symbols: K5B; K78; Kb40; CK-78
• NCBI Protein Information: keratin, type II cytoskeletal 78
• UniProt Protein Name: Keratin, type II cytoskeletal 78
• Protein Family: Keratin
• UniProt Gene Name: KRT78
• UniProt Synonym Gene Names: K5B; KB40; CK-78; K78
• UniProt Entry Name: K2C78_HUMAN

NCBI Description

This gene is a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

KRT78: a member of the type II keratin gene family and encodes a protein with an intermediate filament domain. Keratins are the major structural proteins in epithelial cells, forming a cytoplasmic network of 10 to 12 nm wide intermediate filaments and creating a scaffold that gives cells the ability to withstand mechanical and non-mechanical stresses. The genes of the type II keratin family are located as a gene cluster at 12p13.13. Four pseudogenes of this gene family have been identified. [provided by RefSeq, Jul 2008]

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: extracellular space; keratin filament

Molecular Function: structural molecule activity