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Western Blot (WB) (Western blot analysis of extracts from 293 cells using phospho-JUND-S255 antibody and the same antibody preincubated with blocking peptide.)

Rabbit JUN D Polyclonal Antibody | anti-JunD antibody

JUN D, Phosphorylated (S255) (Jun D Proto-oncogene, JunD, AP-1, Transcription Factor Jun-D) (Biotin)

Gene Names
JUND; AP-1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified by affinity chromatography.
Synonyms
JUN D; Polyclonal Antibody; Phosphorylated (S255) (Jun D Proto-oncogene; JunD; AP-1; Transcription Factor Jun-D) (Biotin); anti-JunD antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, Mouse, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes JUND, phosphorylated S255. Species Crossreactivity: human, mouse, rat
Purity/Purification
Purified by affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Concentration
~1mg/ml (varies by lot)
Applicable Applications for anti-JunD antibody
Immunohistochemistry (IHC), Western Blot (WB)
Application Notes
IHC: 1:50-1:100
WB: 1:500-1:1000
Applications are based on unconjugated antibody.
Immunogen
Phospho specific peptide corresponding to residues surrounding S255 of human JUND.
Conjugate
Biotin
Antigen Modification
Phosphorylated
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western blot analysis of extracts from 293 cells using phospho-JUND-S255 antibody and the same antibody preincubated with blocking peptide.)

Western Blot (WB) (Western blot analysis of extracts from 293 cells using phospho-JUND-S255 antibody and the same antibody preincubated with blocking peptide.)
Product Categories/Family for anti-JunD antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
35,174 Da
NCBI Official Full Name
transcription factor jun-D
NCBI Official Synonym Full Names
jun D proto-oncogene
NCBI Official Symbol
JUND
NCBI Official Synonym Symbols
AP-1
NCBI Protein Information
transcription factor jun-D; JunD-FL isoform; activator protein 1
UniProt Protein Name
Transcription factor jun-D
Protein Family
UniProt Gene Name
JUND
UniProt Entry Name
JUND_HUMAN

NCBI Description

The protein encoded by this intronless gene is a member of the JUN family, and a functional component of the AP1 transcription factor complex. It has been proposed to protect cells from p53-dependent senescence and apoptosis. Alternate translation initiation site usage results in the production of different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

JunD: Transcription factor binding AP-1 sites. Binds DNA as a dimer. Interacts with MEN1; this interaction represses transcriptional activation. Belongs to the bZIP family. Jun subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; protein complex; nucleus; chromatin

Molecular Function: ligand-dependent nuclear receptor binding; protein binding; enzyme binding; sequence-specific DNA binding; double-stranded DNA binding; transcription factor activity

Biological Process: circadian rhythm; regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; response to light stimulus; positive regulation of osteoblast differentiation; response to peptide hormone stimulus; response to mechanical stimulus; response to lipopolysaccharide; positive regulation of transcription from RNA polymerase II promoter; response to organic cyclic substance; aging; osteoblast development

Research Articles on JunD

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Product Notes

The JunD jund (Catalog #AAA6423451) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The JUN D, Phosphorylated (S255) (Jun D Proto-oncogene, JunD, AP-1, Transcription Factor Jun-D) (Biotin) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's JUN D can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB). IHC: 1:50-1:100 WB: 1:500-1:1000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the JunD jund for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "JUN D, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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