Rabbit JUN B Polyclonal Antibody | anti-JUNB antibody

JUN B, Phosphorylated (S79) (AP-1, Jun B proto-oncogene, Transcription Factor Jun-B) (PE)

Cat. Num. AAA6423426

• Gene Names: JUNB; AP-1
• Reactivity: Human, Mouse, Rat
• Applications: Immunofluorescence, Immunohistochemistry
• Purity: Purified by affinity chromatography.
• Synonyms: JUN B; Polyclonal Antibody; Phosphorylated (S79) (AP-1; Jun B proto-oncogene; Transcription Factor Jun-B) (PE); anti-JUNB antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes JUNB, phosphorylated S79. Species Crossreactivity: human, mouse, rat
• Purity/Purification: Purified by affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
• Concentration: ~1mg/ml (varies by lot)

• Applicable Applications for anti-JUNB antibody: Immunofluorescence (IF), Immunohistochemistry (IHC)
• Application Notes: IF: 1:100-1:200; IHC: 1:50-1:100; Applications are based on unconjugated antibody.
• Immunogen: Phospho specific peptide corresponding to residues surrounding S79 of human JUNB.
• Conjugate: PE
• Antigen Modification: Phosphorylated
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Phospho-JUNB-S79 antibody.)

Immunofluorescence (IF)

(Immunofluorescence staining of methanol-fixed Hela cells using Phospho-JUNB-S79 antibody.)

Product Categories/Family for anti-JUNB antibody

Antibodies; Transcription Factors

NCBI and Uniprot Product Information

• NCBI GI #: 4504809
• NCBI GeneID: 3726
• NCBI Accession #: NP_002220.1
• NCBI GenBank Nucleotide #: NM_002229.2
• UniProt Accession #: P17275; Q96GH3
• Molecular Weight: 35,879 Da
• NCBI Official Full Name: transcription factor jun-B
• NCBI Official Synonym Full Names: jun B proto-oncogene
• NCBI Official Symbol: JUNB
• NCBI Official Synonym Symbols: AP-1
• NCBI Protein Information: transcription factor jun-B; activator protein 1
• UniProt Protein Name: Transcription factor jun-B
• Protein Family: Transcription factor
• UniProt Gene Name: JUNB
• UniProt Entry Name: JUNB_HUMAN

Uniprot Description

JunB: Transcription factor involved in regulating gene activity following the primary growth factor response. Binds to the DNA sequence 5'-TGA[CG]TCA-3'. Binds DNA as an homodimer or as an heterodimer with another member of the Jun/Fos family. Interacts with ITCH (via its WW domains). By growth factors. Belongs to the bZIP family. Jun subfamily.

Protein type: Transcription factor; Motility/polarity/chemotaxis; Oncoprotein

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: nucleoplasm; chromatin

Molecular Function: protein binding; DNA binding; transcription coactivator activity; double-stranded DNA binding; transcription factor activity; transcription corepressor activity

Biological Process: response to drug; transcription initiation from RNA polymerase II promoter; response to peptide hormone stimulus; response to light stimulus; response to cAMP; transcription, DNA-dependent; regulation of cell cycle; embryonic process involved in female pregnancy; decidualization; osteoclast differentiation; cellular response to hormone stimulus; trophectodermal cell differentiation; regulation of transcription from RNA polymerase II promoter; osteoblast differentiation; osteoblast proliferation; response to mechanical stimulus; response to cytokine stimulus; transforming growth factor beta receptor signaling pathway; gene expression; positive regulation of transcription from RNA polymerase II promoter; positive regulation of cell differentiation; vasculogenesis; response to progesterone stimulus; response to corticosterone stimulus